A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction

• Autor(a) principal: Freitas,Sueny K. B
• Data de Publicação: 2011
• Outros Autores: Silva,Valdinete Lins da, Araújo,Alberto N, Montenegro,Maria Conceição B. S. M, Reis,Boaventura F, Paim,Ana Paula S
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200013
• topic: amoxicillin; multicommuted flow analysis; photometry; diazo coupling reaction; o-nitroaniline

A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L-1; a detection limit of 5.1 mg L-1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L-1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed.