A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200013 |
Resumo: | A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L-1; a detection limit of 5.1 mg L-1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L-1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed. |
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Journal of the Brazilian Chemical Society (Online) |
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A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reactionamoxicillinmulticommuted flow analysisphotometrydiazo coupling reactiono-nitroanilineA multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L-1; a detection limit of 5.1 mg L-1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L-1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed.Sociedade Brasileira de Química2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200013Journal of the Brazilian Chemical Society v.22 n.2 2011reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532011000200013info:eu-repo/semantics/openAccessFreitas,Sueny K. BSilva,Valdinete Lins daAraújo,Alberto NMontenegro,Maria Conceição B. S. MReis,Boaventura FPaim,Ana Paula Seng2011-02-14T00:00:00Zoai:scielo:S0103-50532011000200013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-02-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
title |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
spellingShingle |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction Freitas,Sueny K. B amoxicillin multicommuted flow analysis photometry diazo coupling reaction o-nitroaniline |
title_short |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
title_full |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
title_fullStr |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
title_full_unstemmed |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
title_sort |
A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction |
author |
Freitas,Sueny K. B |
author_facet |
Freitas,Sueny K. B Silva,Valdinete Lins da Araújo,Alberto N Montenegro,Maria Conceição B. S. M Reis,Boaventura F Paim,Ana Paula S |
author_role |
author |
author2 |
Silva,Valdinete Lins da Araújo,Alberto N Montenegro,Maria Conceição B. S. M Reis,Boaventura F Paim,Ana Paula S |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Freitas,Sueny K. B Silva,Valdinete Lins da Araújo,Alberto N Montenegro,Maria Conceição B. S. M Reis,Boaventura F Paim,Ana Paula S |
dc.subject.por.fl_str_mv |
amoxicillin multicommuted flow analysis photometry diazo coupling reaction o-nitroaniline |
topic |
amoxicillin multicommuted flow analysis photometry diazo coupling reaction o-nitroaniline |
description |
A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L-1; a detection limit of 5.1 mg L-1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L-1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532011000200013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.22 n.2 2011 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318171899822080 |