Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up

Detalhes bibliográficos
Autor(a) principal: Rodenburg,Douglas L.
Data de Publicação: 2016
Outros Autores: Alves,Kamilla, Perera,Wilmer H., Ramsaroop,Taylor, Carvalho,Raquel, McChesney,James D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016000801406
Resumo: Stevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Different stationary phases/columns were used: reverse phase C-18, amino, HILIC (hydrophilic interaction chromatography), Sepaxdiol and Synergi. The advantages and disadvantages of each method for resolving the very polar and less polar elution regions are demonstrated. Strategies for scale up of the purification process of steviol glycosides to make it more economic are also summarized, emphasizing the advantages of using high efficiency normal phase chromatography in relation with reverse phase. Additionally, we also developed an easy and useful way for predicting yields and composition of the pools resulting from the preparative chromatography. The development of these analytical and preparative liquid chromatography techniques allowed the isolation of several steviol glycosides in gram quantities at relatively low cost.
id SBQ-2_a893b9144f43484f10a9a659ca87e077
oai_identifier_str oai:scielo:S0103-50532016000801406
network_acronym_str SBQ-2
network_name_str Journal of the Brazilian Chemical Society (Online)
repository_id_str
spelling Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-UpStevia rebaudianaditerpene glycosideHPLC techniquescale-upenhanced normal phase preparative chromatographyStevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Different stationary phases/columns were used: reverse phase C-18, amino, HILIC (hydrophilic interaction chromatography), Sepaxdiol and Synergi. The advantages and disadvantages of each method for resolving the very polar and less polar elution regions are demonstrated. Strategies for scale up of the purification process of steviol glycosides to make it more economic are also summarized, emphasizing the advantages of using high efficiency normal phase chromatography in relation with reverse phase. Additionally, we also developed an easy and useful way for predicting yields and composition of the pools resulting from the preparative chromatography. The development of these analytical and preparative liquid chromatography techniques allowed the isolation of several steviol glycosides in gram quantities at relatively low cost.Sociedade Brasileira de Química2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016000801406Journal of the Brazilian Chemical Society v.27 n.8 2016reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20160082info:eu-repo/semantics/openAccessRodenburg,Douglas L.Alves,KamillaPerera,Wilmer H.Ramsaroop,TaylorCarvalho,RaquelMcChesney,James D.eng2016-08-19T00:00:00Zoai:scielo:S0103-50532016000801406Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2016-08-19T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
title Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
spellingShingle Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
Rodenburg,Douglas L.
Stevia rebaudiana
diterpene glycoside
HPLC technique
scale-up
enhanced normal phase preparative chromatography
title_short Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
title_full Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
title_fullStr Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
title_full_unstemmed Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
title_sort Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
author Rodenburg,Douglas L.
author_facet Rodenburg,Douglas L.
Alves,Kamilla
Perera,Wilmer H.
Ramsaroop,Taylor
Carvalho,Raquel
McChesney,James D.
author_role author
author2 Alves,Kamilla
Perera,Wilmer H.
Ramsaroop,Taylor
Carvalho,Raquel
McChesney,James D.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rodenburg,Douglas L.
Alves,Kamilla
Perera,Wilmer H.
Ramsaroop,Taylor
Carvalho,Raquel
McChesney,James D.
dc.subject.por.fl_str_mv Stevia rebaudiana
diterpene glycoside
HPLC technique
scale-up
enhanced normal phase preparative chromatography
topic Stevia rebaudiana
diterpene glycoside
HPLC technique
scale-up
enhanced normal phase preparative chromatography
description Stevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Different stationary phases/columns were used: reverse phase C-18, amino, HILIC (hydrophilic interaction chromatography), Sepaxdiol and Synergi. The advantages and disadvantages of each method for resolving the very polar and less polar elution regions are demonstrated. Strategies for scale up of the purification process of steviol glycosides to make it more economic are also summarized, emphasizing the advantages of using high efficiency normal phase chromatography in relation with reverse phase. Additionally, we also developed an easy and useful way for predicting yields and composition of the pools resulting from the preparative chromatography. The development of these analytical and preparative liquid chromatography techniques allowed the isolation of several steviol glycosides in gram quantities at relatively low cost.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016000801406
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016000801406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20160082
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.27 n.8 2016
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
_version_ 1750318178683060224