A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101255 |
Resumo: | We developed a colorimetric and fluorescence dual-signal sensor for detecting lipase activity based on methyl thioglycolate (MT) functionalized gold nanoparticles (MT-AuNPs) and CdS quantum dots (CdS QDs). It is the first time that was developed a dual-signal sensing strategy for lipase activity based on the inner-filter effect (IFE) between MT-AuNPs and CdS QDs. This probe system (MT AuNPs + CdS QDs) is sensitive and has selective response to the concentration of the lipase, and MT-AuNPs and CdS QDs played as transducer and fluorescer, respectively. The addition of lipase triggered the accumulation and change of color in CdS QDs and MT-AuNPs solution, it also makes the fluorescence intesity of CdS QDs significantly recover. The limit of detection (LOD) was as low as 0.039 μg mL-1 for colorimetric detection as well as 0.012 μg mL-1 for the fluorescence method. This method has been successfully used in the detection of commercial lipases. We believe it would open up a new path for the sensitive and high throughput lipase assay using nanobiosensor. |
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Journal of the Brazilian Chemical Society (Online) |
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A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effectlipasedual-signalbiosensorinner filter effectWe developed a colorimetric and fluorescence dual-signal sensor for detecting lipase activity based on methyl thioglycolate (MT) functionalized gold nanoparticles (MT-AuNPs) and CdS quantum dots (CdS QDs). It is the first time that was developed a dual-signal sensing strategy for lipase activity based on the inner-filter effect (IFE) between MT-AuNPs and CdS QDs. This probe system (MT AuNPs + CdS QDs) is sensitive and has selective response to the concentration of the lipase, and MT-AuNPs and CdS QDs played as transducer and fluorescer, respectively. The addition of lipase triggered the accumulation and change of color in CdS QDs and MT-AuNPs solution, it also makes the fluorescence intesity of CdS QDs significantly recover. The limit of detection (LOD) was as low as 0.039 μg mL-1 for colorimetric detection as well as 0.012 μg mL-1 for the fluorescence method. This method has been successfully used in the detection of commercial lipases. We believe it would open up a new path for the sensitive and high throughput lipase assay using nanobiosensor.Sociedade Brasileira de Química2022-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101255Journal of the Brazilian Chemical Society v.33 n.11 2022reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20220055info:eu-repo/semantics/openAccessDu,TingZhang,FuJiang,LingTian,Danbieng2022-10-21T00:00:00Zoai:scielo:S0103-50532022001101255Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2022-10-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
title |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
spellingShingle |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect Du,Ting lipase dual-signal biosensor inner filter effect |
title_short |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
title_full |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
title_fullStr |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
title_full_unstemmed |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
title_sort |
A Colorimetric and Fluorescent Dual-Signal Sensor for Detecting Lipase Activity Based on Inner Filter Effect |
author |
Du,Ting |
author_facet |
Du,Ting Zhang,Fu Jiang,Ling Tian,Danbi |
author_role |
author |
author2 |
Zhang,Fu Jiang,Ling Tian,Danbi |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Du,Ting Zhang,Fu Jiang,Ling Tian,Danbi |
dc.subject.por.fl_str_mv |
lipase dual-signal biosensor inner filter effect |
topic |
lipase dual-signal biosensor inner filter effect |
description |
We developed a colorimetric and fluorescence dual-signal sensor for detecting lipase activity based on methyl thioglycolate (MT) functionalized gold nanoparticles (MT-AuNPs) and CdS quantum dots (CdS QDs). It is the first time that was developed a dual-signal sensing strategy for lipase activity based on the inner-filter effect (IFE) between MT-AuNPs and CdS QDs. This probe system (MT AuNPs + CdS QDs) is sensitive and has selective response to the concentration of the lipase, and MT-AuNPs and CdS QDs played as transducer and fluorescer, respectively. The addition of lipase triggered the accumulation and change of color in CdS QDs and MT-AuNPs solution, it also makes the fluorescence intesity of CdS QDs significantly recover. The limit of detection (LOD) was as low as 0.039 μg mL-1 for colorimetric detection as well as 0.012 μg mL-1 for the fluorescence method. This method has been successfully used in the detection of commercial lipases. We believe it would open up a new path for the sensitive and high throughput lipase assay using nanobiosensor. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101255 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20220055 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.33 n.11 2022 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318185018556416 |