Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000600006 |
Resumo: | Microwave-induced combustion system was coupled to a metallic flame furnace (FF) atomic absorption spectrometer and applied for cadmium determination in blood. Lyophilized blood samples were prepared as pellets, positioned on a quartz holder and introduced into a glass chamber used for the combustion step. The glass chamber was coupled to the metallic flame furnace by using polytetrafluoroethylene and quartz tubes. Ignition was performed by microwave radiation using ammonium nitrate solution added to a small piece of previously cleaned paper. Oxygen was used to assist the sample combustion and also to transport combustion products up to the FF heated by an air/acetylene flame. High purity graphite powder was mixed with samples before pellets preparation. The volume of NH4NO3 solution, oxygen flow-rate, flame stoichiometry, sample holder type and the sample mass range were evaluated. Calibration step was feasible using adsorbed reference solution in pelletized high purity graphite avoiding the use of certified reference material (CRM). Sample masses up to 56 µg could be used allowing quantification limit of 0.018 µg g-1 and characteristic mass of 50 pg Cd. Low values for blanks were obtained (integrated absorbance lower than 0.01 s) and results were considered satisfactory regarding to both accuracy (agreement with results using CRMs between 95 to 99%), and precision (relative standard deviation < 12%). Up to 15 determinations can be performed per hour including the weighing step. |
id |
SBQ-2_b3b524b8a18ec1758635fa71cd53756f |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532010000600006 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometryflame furnace atomic absorption spectrometrymicrowave induced combustioncadmium determinationsolid samplingblood analysisMicrowave-induced combustion system was coupled to a metallic flame furnace (FF) atomic absorption spectrometer and applied for cadmium determination in blood. Lyophilized blood samples were prepared as pellets, positioned on a quartz holder and introduced into a glass chamber used for the combustion step. The glass chamber was coupled to the metallic flame furnace by using polytetrafluoroethylene and quartz tubes. Ignition was performed by microwave radiation using ammonium nitrate solution added to a small piece of previously cleaned paper. Oxygen was used to assist the sample combustion and also to transport combustion products up to the FF heated by an air/acetylene flame. High purity graphite powder was mixed with samples before pellets preparation. The volume of NH4NO3 solution, oxygen flow-rate, flame stoichiometry, sample holder type and the sample mass range were evaluated. Calibration step was feasible using adsorbed reference solution in pelletized high purity graphite avoiding the use of certified reference material (CRM). Sample masses up to 56 µg could be used allowing quantification limit of 0.018 µg g-1 and characteristic mass of 50 pg Cd. Low values for blanks were obtained (integrated absorbance lower than 0.01 s) and results were considered satisfactory regarding to both accuracy (agreement with results using CRMs between 95 to 99%), and precision (relative standard deviation < 12%). Up to 15 determinations can be performed per hour including the weighing step.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000600006Journal of the Brazilian Chemical Society v.21 n.6 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532010000600006info:eu-repo/semantics/openAccessHoehne,LucéliaBartz,Fabiane R.Bizzi,Cezar A.Paniz,José N. G.Dressler,Valderi L.Flores,Érico M. de Moraeseng2010-07-19T00:00:00Zoai:scielo:S0103-50532010000600006Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2010-07-19T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
title |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
spellingShingle |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry Hoehne,Lucélia flame furnace atomic absorption spectrometry microwave induced combustion cadmium determination solid sampling blood analysis |
title_short |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
title_full |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
title_fullStr |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
title_full_unstemmed |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
title_sort |
Determination of Cd in blood by microwave-induced combustion coupled to flame furnace atomic absorption spectrometry |
author |
Hoehne,Lucélia |
author_facet |
Hoehne,Lucélia Bartz,Fabiane R. Bizzi,Cezar A. Paniz,José N. G. Dressler,Valderi L. Flores,Érico M. de Moraes |
author_role |
author |
author2 |
Bartz,Fabiane R. Bizzi,Cezar A. Paniz,José N. G. Dressler,Valderi L. Flores,Érico M. de Moraes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Hoehne,Lucélia Bartz,Fabiane R. Bizzi,Cezar A. Paniz,José N. G. Dressler,Valderi L. Flores,Érico M. de Moraes |
dc.subject.por.fl_str_mv |
flame furnace atomic absorption spectrometry microwave induced combustion cadmium determination solid sampling blood analysis |
topic |
flame furnace atomic absorption spectrometry microwave induced combustion cadmium determination solid sampling blood analysis |
description |
Microwave-induced combustion system was coupled to a metallic flame furnace (FF) atomic absorption spectrometer and applied for cadmium determination in blood. Lyophilized blood samples were prepared as pellets, positioned on a quartz holder and introduced into a glass chamber used for the combustion step. The glass chamber was coupled to the metallic flame furnace by using polytetrafluoroethylene and quartz tubes. Ignition was performed by microwave radiation using ammonium nitrate solution added to a small piece of previously cleaned paper. Oxygen was used to assist the sample combustion and also to transport combustion products up to the FF heated by an air/acetylene flame. High purity graphite powder was mixed with samples before pellets preparation. The volume of NH4NO3 solution, oxygen flow-rate, flame stoichiometry, sample holder type and the sample mass range were evaluated. Calibration step was feasible using adsorbed reference solution in pelletized high purity graphite avoiding the use of certified reference material (CRM). Sample masses up to 56 µg could be used allowing quantification limit of 0.018 µg g-1 and characteristic mass of 50 pg Cd. Low values for blanks were obtained (integrated absorbance lower than 0.01 s) and results were considered satisfactory regarding to both accuracy (agreement with results using CRMs between 95 to 99%), and precision (relative standard deviation < 12%). Up to 15 determinations can be performed per hour including the weighing step. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000600006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000600006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532010000600006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.21 n.6 2010 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318171025309696 |