Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD

Detalhes bibliográficos
Autor(a) principal: Bauer,Luciana C.
Data de Publicação: 2014
Outros Autores: Santana,Débora de A., Macedo,Marianne dos S., Torres,Alexandre G., Souza,Nilson E. de, Simionato,Julliana I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000100020
Resumo: The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.
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spelling Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DADdirect saponification7-ketocholesterol25-hydroxycholesterolliquid chromatographydairy fatThe aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.Sociedade Brasileira de Química2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000100020Journal of the Brazilian Chemical Society v.25 n.1 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130283info:eu-repo/semantics/openAccessBauer,Luciana C.Santana,Débora de A.Macedo,Marianne dos S.Torres,Alexandre G.Souza,Nilson E. deSimionato,Julliana I.eng2014-01-28T00:00:00Zoai:scielo:S0103-50532014000100020Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-01-28T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
title Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
spellingShingle Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
Bauer,Luciana C.
direct saponification
7-ketocholesterol
25-hydroxycholesterol
liquid chromatography
dairy fat
title_short Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
title_full Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
title_fullStr Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
title_full_unstemmed Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
title_sort Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
author Bauer,Luciana C.
author_facet Bauer,Luciana C.
Santana,Débora de A.
Macedo,Marianne dos S.
Torres,Alexandre G.
Souza,Nilson E. de
Simionato,Julliana I.
author_role author
author2 Santana,Débora de A.
Macedo,Marianne dos S.
Torres,Alexandre G.
Souza,Nilson E. de
Simionato,Julliana I.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bauer,Luciana C.
Santana,Débora de A.
Macedo,Marianne dos S.
Torres,Alexandre G.
Souza,Nilson E. de
Simionato,Julliana I.
dc.subject.por.fl_str_mv direct saponification
7-ketocholesterol
25-hydroxycholesterol
liquid chromatography
dairy fat
topic direct saponification
7-ketocholesterol
25-hydroxycholesterol
liquid chromatography
dairy fat
description The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000100020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20130283
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.25 n.1 2014
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
_version_ 1750318175696715776

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