Evaluating intracellular redox status in L02 cells on microchip

Detalhes bibliográficos
Autor(a) principal: Xu,Chunxiu
Data de Publicação: 2013
Outros Autores: Cai,Longfei
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000300005
Resumo: A novel microfluidic device integrated with continuous introduction of individual cells and detection of intracellular reducing species by laser induced fluorescence (LIF) detector is reported. A single channel with one sheath-flow channel located on each side of the sampling channel was designed. The intracellular reducing species were derivatized by a newly synthesized 2,2,6,6-tetramethyl-piperidine-1-oxyl (TEMPO)-based fluorescent probe. The labeled cells were hydrodynamically focused by sheath-flow streams and sequentially introduced into the sampling channel under hydrostatic pressure generated by adjusting liquid levels in the reservoirs. Intracellular reducing level in intact living L02 cells was detected by LIF without cytolysis. Upon stimulation with 100 µmol L-1 hydrogen peroxide for 30 min, the intracellular reducing level decreased in response to oxidative stress. A throughput of 41-44 cells min-1 was obtained.
id SBQ-2_cdc38a90018e8a13bc9e14524b3961a6
oai_identifier_str oai:scielo:S0103-50532013000300005
network_acronym_str SBQ-2
network_name_str Journal of the Brazilian Chemical Society (Online)
repository_id_str
spelling Evaluating intracellular redox status in L02 cells on microchipsingle-cell analysismicrofluidicshigh-throughputA novel microfluidic device integrated with continuous introduction of individual cells and detection of intracellular reducing species by laser induced fluorescence (LIF) detector is reported. A single channel with one sheath-flow channel located on each side of the sampling channel was designed. The intracellular reducing species were derivatized by a newly synthesized 2,2,6,6-tetramethyl-piperidine-1-oxyl (TEMPO)-based fluorescent probe. The labeled cells were hydrodynamically focused by sheath-flow streams and sequentially introduced into the sampling channel under hydrostatic pressure generated by adjusting liquid levels in the reservoirs. Intracellular reducing level in intact living L02 cells was detected by LIF without cytolysis. Upon stimulation with 100 µmol L-1 hydrogen peroxide for 30 min, the intracellular reducing level decreased in response to oxidative stress. A throughput of 41-44 cells min-1 was obtained.Sociedade Brasileira de Química2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000300005Journal of the Brazilian Chemical Society v.24 n.3 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130050info:eu-repo/semantics/openAccessXu,ChunxiuCai,Longfeieng2013-05-24T00:00:00Zoai:scielo:S0103-50532013000300005Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-05-24T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Evaluating intracellular redox status in L02 cells on microchip
title Evaluating intracellular redox status in L02 cells on microchip
spellingShingle Evaluating intracellular redox status in L02 cells on microchip
Xu,Chunxiu
single-cell analysis
microfluidics
high-throughput
title_short Evaluating intracellular redox status in L02 cells on microchip
title_full Evaluating intracellular redox status in L02 cells on microchip
title_fullStr Evaluating intracellular redox status in L02 cells on microchip
title_full_unstemmed Evaluating intracellular redox status in L02 cells on microchip
title_sort Evaluating intracellular redox status in L02 cells on microchip
author Xu,Chunxiu
author_facet Xu,Chunxiu
Cai,Longfei
author_role author
author2 Cai,Longfei
author2_role author
dc.contributor.author.fl_str_mv Xu,Chunxiu
Cai,Longfei
dc.subject.por.fl_str_mv single-cell analysis
microfluidics
high-throughput
topic single-cell analysis
microfluidics
high-throughput
description A novel microfluidic device integrated with continuous introduction of individual cells and detection of intracellular reducing species by laser induced fluorescence (LIF) detector is reported. A single channel with one sheath-flow channel located on each side of the sampling channel was designed. The intracellular reducing species were derivatized by a newly synthesized 2,2,6,6-tetramethyl-piperidine-1-oxyl (TEMPO)-based fluorescent probe. The labeled cells were hydrodynamically focused by sheath-flow streams and sequentially introduced into the sampling channel under hydrostatic pressure generated by adjusting liquid levels in the reservoirs. Intracellular reducing level in intact living L02 cells was detected by LIF without cytolysis. Upon stimulation with 100 µmol L-1 hydrogen peroxide for 30 min, the intracellular reducing level decreased in response to oxidative stress. A throughput of 41-44 cells min-1 was obtained.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000300005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20130050
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.24 n.3 2013
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
_version_ 1750318174498193408

Registros relacionados