Detection of retinoic acid receptor complex using mass spectrometry

Detalhes bibliográficos
Autor(a) principal: Zhang,Lin
Data de Publicação: 2007
Outros Autores: Song,Zeqing
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000300005
Resumo: Retinoic acid receptor is a transcription factor in the cell nucleus that binds retinoic acid. The ligand binding domain of human gamma retinoic acid receptor, its complex with retinoic acid and its interaction with retinoic acid binding protein I, were studied by means of mass spectrometric methods. The pathway of delivering retinoic acid from donor to acceptor was monitored online. No ternary complex involving retinoic acid and the two proteins was observed, indicating that the transport of retinoic acid from retinoic acid binding protein I to its receptor may follow a two step mechanism, which involves dissociation of retinoic acid from the donor protein into the aqueous phase, followed by association with the acceptor.
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spelling Detection of retinoic acid receptor complex using mass spectrometryretinoic acid receptorretinoic acid binding proteinelectrospray ionization mass spectrometrynoncovalent complexRetinoic acid receptor is a transcription factor in the cell nucleus that binds retinoic acid. The ligand binding domain of human gamma retinoic acid receptor, its complex with retinoic acid and its interaction with retinoic acid binding protein I, were studied by means of mass spectrometric methods. The pathway of delivering retinoic acid from donor to acceptor was monitored online. No ternary complex involving retinoic acid and the two proteins was observed, indicating that the transport of retinoic acid from retinoic acid binding protein I to its receptor may follow a two step mechanism, which involves dissociation of retinoic acid from the donor protein into the aqueous phase, followed by association with the acceptor.Sociedade Brasileira de Química2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000300005Journal of the Brazilian Chemical Society v.18 n.3 2007reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532007000300005info:eu-repo/semantics/openAccessZhang,LinSong,Zeqingeng2007-06-28T00:00:00Zoai:scielo:S0103-50532007000300005Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2007-06-28T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Detection of retinoic acid receptor complex using mass spectrometry
title Detection of retinoic acid receptor complex using mass spectrometry
spellingShingle Detection of retinoic acid receptor complex using mass spectrometry
Zhang,Lin
retinoic acid receptor
retinoic acid binding protein
electrospray ionization mass spectrometry
noncovalent complex
title_short Detection of retinoic acid receptor complex using mass spectrometry
title_full Detection of retinoic acid receptor complex using mass spectrometry
title_fullStr Detection of retinoic acid receptor complex using mass spectrometry
title_full_unstemmed Detection of retinoic acid receptor complex using mass spectrometry
title_sort Detection of retinoic acid receptor complex using mass spectrometry
author Zhang,Lin
author_facet Zhang,Lin
Song,Zeqing
author_role author
author2 Song,Zeqing
author2_role author
dc.contributor.author.fl_str_mv Zhang,Lin
Song,Zeqing
dc.subject.por.fl_str_mv retinoic acid receptor
retinoic acid binding protein
electrospray ionization mass spectrometry
noncovalent complex
topic retinoic acid receptor
retinoic acid binding protein
electrospray ionization mass spectrometry
noncovalent complex
description Retinoic acid receptor is a transcription factor in the cell nucleus that binds retinoic acid. The ligand binding domain of human gamma retinoic acid receptor, its complex with retinoic acid and its interaction with retinoic acid binding protein I, were studied by means of mass spectrometric methods. The pathway of delivering retinoic acid from donor to acceptor was monitored online. No ternary complex involving retinoic acid and the two proteins was observed, indicating that the transport of retinoic acid from retinoic acid binding protein I to its receptor may follow a two step mechanism, which involves dissociation of retinoic acid from the donor protein into the aqueous phase, followed by association with the acceptor.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000300005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532007000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.18 n.3 2007
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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