HPLC method to assay total saponins in Ilex paraguariensis aqueous extract

Detalhes bibliográficos
Autor(a) principal: Gnoatto,Simone C.B.
Data de Publicação: 2005
Outros Autores: Schenkel,Eloir P., Bassani,Valquiria L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007
Resumo: Ilex paraguariensis St. Hilaire is a South American tree from which leaves and twigs are used to prepare a commonly consumed tea in several South American countries. The plant is known as "erva-mate" in Portuguese or "yerba mate" in Spanish. Considering the potential use of the saponins as surfactant as well as its therapeutic potential, the present work was designed to propose extraction and quantitation methods for the saponins present in Ilex paraguariensis. The saponins were extracted by decoction, hydrolyzed and quantified by a HPLC method with UV detection. The saponins concentration was expressed in ursolic acid (total saponins). The method showed linearity for ursolic acid in the range of 13.5 to 135 µg mL-1. The aqueous extract presented total saponins concentration of 352 µg mL-1. The results also suggest the possibility of use of similar method for assaying triterpenoid saponins in other plants.
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spelling HPLC method to assay total saponins in Ilex paraguariensis aqueous extractHPLCsaponinsIlex paraguariensiserva-mateursolic acidsaponin quantitationIlex paraguariensis St. Hilaire is a South American tree from which leaves and twigs are used to prepare a commonly consumed tea in several South American countries. The plant is known as "erva-mate" in Portuguese or "yerba mate" in Spanish. Considering the potential use of the saponins as surfactant as well as its therapeutic potential, the present work was designed to propose extraction and quantitation methods for the saponins present in Ilex paraguariensis. The saponins were extracted by decoction, hydrolyzed and quantified by a HPLC method with UV detection. The saponins concentration was expressed in ursolic acid (total saponins). The method showed linearity for ursolic acid in the range of 13.5 to 135 µg mL-1. The aqueous extract presented total saponins concentration of 352 µg mL-1. The results also suggest the possibility of use of similar method for assaying triterpenoid saponins in other plants.Sociedade Brasileira de Química2005-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007Journal of the Brazilian Chemical Society v.16 n.4 2005reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532005000500007info:eu-repo/semantics/openAccessGnoatto,Simone C.B.Schenkel,Eloir P.Bassani,Valquiria L.eng2005-08-25T00:00:00Zoai:scielo:S0103-50532005000500007Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2005-08-25T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
title HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
spellingShingle HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
Gnoatto,Simone C.B.
HPLC
saponins
Ilex paraguariensis
erva-mate
ursolic acid
saponin quantitation
title_short HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
title_full HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
title_fullStr HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
title_full_unstemmed HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
title_sort HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
author Gnoatto,Simone C.B.
author_facet Gnoatto,Simone C.B.
Schenkel,Eloir P.
Bassani,Valquiria L.
author_role author
author2 Schenkel,Eloir P.
Bassani,Valquiria L.
author2_role author
author
dc.contributor.author.fl_str_mv Gnoatto,Simone C.B.
Schenkel,Eloir P.
Bassani,Valquiria L.
dc.subject.por.fl_str_mv HPLC
saponins
Ilex paraguariensis
erva-mate
ursolic acid
saponin quantitation
topic HPLC
saponins
Ilex paraguariensis
erva-mate
ursolic acid
saponin quantitation
description Ilex paraguariensis St. Hilaire is a South American tree from which leaves and twigs are used to prepare a commonly consumed tea in several South American countries. The plant is known as "erva-mate" in Portuguese or "yerba mate" in Spanish. Considering the potential use of the saponins as surfactant as well as its therapeutic potential, the present work was designed to propose extraction and quantitation methods for the saponins present in Ilex paraguariensis. The saponins were extracted by decoction, hydrolyzed and quantified by a HPLC method with UV detection. The saponins concentration was expressed in ursolic acid (total saponins). The method showed linearity for ursolic acid in the range of 13.5 to 135 µg mL-1. The aqueous extract presented total saponins concentration of 352 µg mL-1. The results also suggest the possibility of use of similar method for assaying triterpenoid saponins in other plants.
publishDate 2005
dc.date.none.fl_str_mv 2005-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532005000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.16 n.4 2005
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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