COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236 |
Resumo: | Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy. |
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COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOSendocytosisintracellular traffickingnanoparticles, nanotoxicologynano-cell interactionsAlthough in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy.Sociedade Brasileira de Química2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236Química Nova v.39 n.10 2016reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20160146info:eu-repo/semantics/openAccessRadaic,AllanPugliese,Gustavo OliveiraCampese,Guilherme CapiraçoPessine,Francisco Benedito TeixeiraJesus,Marcelo Bispo depor2016-12-20T00:00:00Zoai:scielo:S0100-40422016001001236Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2016-12-20T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
title |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
spellingShingle |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS Radaic,Allan endocytosis intracellular trafficking nanoparticles, nanotoxicology nano-cell interactions |
title_short |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
title_full |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
title_fullStr |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
title_full_unstemmed |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
title_sort |
COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS |
author |
Radaic,Allan |
author_facet |
Radaic,Allan Pugliese,Gustavo Oliveira Campese,Guilherme Capiraço Pessine,Francisco Benedito Teixeira Jesus,Marcelo Bispo de |
author_role |
author |
author2 |
Pugliese,Gustavo Oliveira Campese,Guilherme Capiraço Pessine,Francisco Benedito Teixeira Jesus,Marcelo Bispo de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Radaic,Allan Pugliese,Gustavo Oliveira Campese,Guilherme Capiraço Pessine,Francisco Benedito Teixeira Jesus,Marcelo Bispo de |
dc.subject.por.fl_str_mv |
endocytosis intracellular trafficking nanoparticles, nanotoxicology nano-cell interactions |
topic |
endocytosis intracellular trafficking nanoparticles, nanotoxicology nano-cell interactions |
description |
Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20160146 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.39 n.10 2016 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318117938003968 |