COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS

Detalhes bibliográficos
Autor(a) principal: Radaic,Allan
Data de Publicação: 2016
Outros Autores: Pugliese,Gustavo Oliveira, Campese,Guilherme Capiraço, Pessine,Francisco Benedito Teixeira, Jesus,Marcelo Bispo de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236
Resumo: Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy.
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spelling COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOSendocytosisintracellular traffickingnanoparticles, nanotoxicologynano-cell interactionsAlthough in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy.Sociedade Brasileira de Química2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236Química Nova v.39 n.10 2016reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20160146info:eu-repo/semantics/openAccessRadaic,AllanPugliese,Gustavo OliveiraCampese,Guilherme CapiraçoPessine,Francisco Benedito TeixeiraJesus,Marcelo Bispo depor2016-12-20T00:00:00Zoai:scielo:S0100-40422016001001236Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2016-12-20T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
title COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
spellingShingle COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
Radaic,Allan
endocytosis
intracellular trafficking
nanoparticles, nanotoxicology
nano-cell interactions
title_short COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
title_full COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
title_fullStr COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
title_full_unstemmed COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
title_sort COMO ESTUDAR INTERAÇÕES ENTRE NANOPARTÍCULAS E SISTEMAS BIOLÓGICOS
author Radaic,Allan
author_facet Radaic,Allan
Pugliese,Gustavo Oliveira
Campese,Guilherme Capiraço
Pessine,Francisco Benedito Teixeira
Jesus,Marcelo Bispo de
author_role author
author2 Pugliese,Gustavo Oliveira
Campese,Guilherme Capiraço
Pessine,Francisco Benedito Teixeira
Jesus,Marcelo Bispo de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Radaic,Allan
Pugliese,Gustavo Oliveira
Campese,Guilherme Capiraço
Pessine,Francisco Benedito Teixeira
Jesus,Marcelo Bispo de
dc.subject.por.fl_str_mv endocytosis
intracellular trafficking
nanoparticles, nanotoxicology
nano-cell interactions
topic endocytosis
intracellular trafficking
nanoparticles, nanotoxicology
nano-cell interactions
description Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016001001236
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.21577/0100-4042.20160146
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.39 n.10 2016
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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