Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café

Detalhes bibliográficos
Autor(a) principal: Martins,Ana Carolina C. L.
Data de Publicação: 2010
Outros Autores: Silva,Tarliane M., Gloria,M. Beatriz A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016
Resumo: Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.
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spelling Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em cafécoffeeserotonintryptophanEpidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016Química Nova v.33 n.2 2010reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422010000200016info:eu-repo/semantics/openAccessMartins,Ana Carolina C. L.Silva,Tarliane M.Gloria,M. Beatriz A.por2010-03-12T00:00:00Zoai:scielo:S0100-40422010000200016Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2010-03-12T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
title Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
spellingShingle Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
Martins,Ana Carolina C. L.
coffee
serotonin
tryptophan
title_short Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
title_full Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
title_fullStr Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
title_full_unstemmed Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
title_sort Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café
author Martins,Ana Carolina C. L.
author_facet Martins,Ana Carolina C. L.
Silva,Tarliane M.
Gloria,M. Beatriz A.
author_role author
author2 Silva,Tarliane M.
Gloria,M. Beatriz A.
author2_role author
author
dc.contributor.author.fl_str_mv Martins,Ana Carolina C. L.
Silva,Tarliane M.
Gloria,M. Beatriz A.
dc.subject.por.fl_str_mv coffee
serotonin
tryptophan
topic coffee
serotonin
tryptophan
description Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200016
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422010000200016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.33 n.2 2010
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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