Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno

Detalhes bibliográficos
Autor(a) principal: Oliveira,Lenise G. de
Data de Publicação: 2014
Outros Autores: Kurz,Marcia H. S., Gonçalves,Fábio F., Barreto,Fabiano, Rübensam,Gabriel, Hoff,Rodrigo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014
Resumo: A procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value.
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spelling Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suínosedativesB-blockersswine kidneyA procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value.Sociedade Brasileira de Química2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014Química Nova v.37 n.8 2014reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20140185info:eu-repo/semantics/openAccessOliveira,Lenise G. deKurz,Marcia H. S.Gonçalves,Fábio F.Barreto,FabianoRübensam,GabrielHoff,Rodrigopor2019-06-28T00:00:00Zoai:scielo:S0100-40422014000800014Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-06-28T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
title Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
spellingShingle Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
Oliveira,Lenise G. de
sedatives
B-blockers
swine kidney
title_short Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
title_full Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
title_fullStr Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
title_full_unstemmed Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
title_sort Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
author Oliveira,Lenise G. de
author_facet Oliveira,Lenise G. de
Kurz,Marcia H. S.
Gonçalves,Fábio F.
Barreto,Fabiano
Rübensam,Gabriel
Hoff,Rodrigo
author_role author
author2 Kurz,Marcia H. S.
Gonçalves,Fábio F.
Barreto,Fabiano
Rübensam,Gabriel
Hoff,Rodrigo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Lenise G. de
Kurz,Marcia H. S.
Gonçalves,Fábio F.
Barreto,Fabiano
Rübensam,Gabriel
Hoff,Rodrigo
dc.subject.por.fl_str_mv sedatives
B-blockers
swine kidney
topic sedatives
B-blockers
swine kidney
description A procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.5935/0100-4042.20140185
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.37 n.8 2014
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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