Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014 |
Resumo: | A procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value. |
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Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suínosedativesB-blockersswine kidneyA procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value.Sociedade Brasileira de Química2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014Química Nova v.37 n.8 2014reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20140185info:eu-repo/semantics/openAccessOliveira,Lenise G. deKurz,Marcia H. S.Gonçalves,Fábio F.Barreto,FabianoRübensam,GabrielHoff,Rodrigopor2019-06-28T00:00:00Zoai:scielo:S0100-40422014000800014Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-06-28T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
title |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
spellingShingle |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno Oliveira,Lenise G. de sedatives B-blockers swine kidney |
title_short |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
title_full |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
title_fullStr |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
title_full_unstemmed |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
title_sort |
Desenvolvimento de procedimento de extração para análise de sedativos e B-bloqueadores em rim suíno |
author |
Oliveira,Lenise G. de |
author_facet |
Oliveira,Lenise G. de Kurz,Marcia H. S. Gonçalves,Fábio F. Barreto,Fabiano Rübensam,Gabriel Hoff,Rodrigo |
author_role |
author |
author2 |
Kurz,Marcia H. S. Gonçalves,Fábio F. Barreto,Fabiano Rübensam,Gabriel Hoff,Rodrigo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Lenise G. de Kurz,Marcia H. S. Gonçalves,Fábio F. Barreto,Fabiano Rübensam,Gabriel Hoff,Rodrigo |
dc.subject.por.fl_str_mv |
sedatives B-blockers swine kidney |
topic |
sedatives B-blockers swine kidney |
description |
A procedure was developed for determination of 5 sedatives and 14 β-blockers in swine kidney and subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Three different procedures for extraction were tested, evaluated through recovery studies. The procedure using acetonitrile for extraction and cleanup with freezing at low temperature and dispersive solid phase extraction using 500 mg celite® 545 before the concentration step presented the better results. The dried samples were redissolved with methanol and analyzed using a LC-MS/MS system with electrospray ionization (ESI) operating in positive MRM mode. The recovery values for this procedure were in the 75-88% range. The robustness of the method was tested against small variations. The method was used to analyze carazolol, azaperone and azaperol in collaborative assay, obtaining results close to designed value. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000800014 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.5935/0100-4042.20140185 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.37 n.8 2014 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318116255039488 |