CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019000400405 |
Resumo: | Colletotrichum gloeosporioides biomass was submitted to ethanolic extraction (78 ºC, 12 h) to remove low molecular weight compounds followed by hot aqueous extraction (100 ºC, 4 h, 4x) to obtain polysaccharides. Consecutive freezing/thawing treatments of the aqueous extract yielded a soluble fraction in cold water that was separated on five components (PI to PV) by gel exclusion chromatography on Sepharose CL-6B. The PIV was homogeneous on HPSEC/RID, with apparent MW of 2.8 x 104 g mol-1, Mw/Mn 1.17, 100% of total sugar. The PIV was composed by galactose (61%), glucose (34%) and mannose (5%). 1H and 13C NMR analysis mono- and two-dimensional, homo- (COSY) and heteronuclear (HSQC and HMBC) showed that PIV is a heteropolysacharide with a main chain constituted by β-D-galactofuranosidic units (1→5) and (1→6) linked with some of the β-D-galactofuranosidic (1→6) units substituted in O-3 by α-D-glucopiranosidic residues. Cytotoxic assays (MTT) indicated that the viability of CHO-K1 cells, when exposed to different heteropolysaccharide concentrations, did not show significant differences when compared to the viability of the negative control (CN), in the experimental times of 24 and 48 hours. These results suggest the possibility of new cytotoxic assays in other normal cell lines as well as in tumor cells. |
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CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioidesheteropolysaccharideHPSEC/RIDHSQCMTT assayColletotrichum gloeosporioides biomass was submitted to ethanolic extraction (78 ºC, 12 h) to remove low molecular weight compounds followed by hot aqueous extraction (100 ºC, 4 h, 4x) to obtain polysaccharides. Consecutive freezing/thawing treatments of the aqueous extract yielded a soluble fraction in cold water that was separated on five components (PI to PV) by gel exclusion chromatography on Sepharose CL-6B. The PIV was homogeneous on HPSEC/RID, with apparent MW of 2.8 x 104 g mol-1, Mw/Mn 1.17, 100% of total sugar. The PIV was composed by galactose (61%), glucose (34%) and mannose (5%). 1H and 13C NMR analysis mono- and two-dimensional, homo- (COSY) and heteronuclear (HSQC and HMBC) showed that PIV is a heteropolysacharide with a main chain constituted by β-D-galactofuranosidic units (1→5) and (1→6) linked with some of the β-D-galactofuranosidic (1→6) units substituted in O-3 by α-D-glucopiranosidic residues. Cytotoxic assays (MTT) indicated that the viability of CHO-K1 cells, when exposed to different heteropolysaccharide concentrations, did not show significant differences when compared to the viability of the negative control (CN), in the experimental times of 24 and 48 hours. These results suggest the possibility of new cytotoxic assays in other normal cell lines as well as in tumor cells.Sociedade Brasileira de Química2019-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019000400405Química Nova v.42 n.4 2019reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170347info:eu-repo/semantics/openAccessAlexandre,Samara M. A.Silva,Maria de Lourdes Corradi daVasconcelos,Ana F. D.Cavalcante,Dalita G. S. M.Job,Aldo E.Ferreira,Luciana G.Noseda,Miguel D.Duarte,Maria E. R.por2019-05-21T00:00:00Zoai:scielo:S0100-40422019000400405Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-05-21T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
title |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
spellingShingle |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides Alexandre,Samara M. A. heteropolysaccharide HPSEC/RID HSQC MTT assay |
title_short |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
title_full |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
title_fullStr |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
title_full_unstemmed |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
title_sort |
CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides |
author |
Alexandre,Samara M. A. |
author_facet |
Alexandre,Samara M. A. Silva,Maria de Lourdes Corradi da Vasconcelos,Ana F. D. Cavalcante,Dalita G. S. M. Job,Aldo E. Ferreira,Luciana G. Noseda,Miguel D. Duarte,Maria E. R. |
author_role |
author |
author2 |
Silva,Maria de Lourdes Corradi da Vasconcelos,Ana F. D. Cavalcante,Dalita G. S. M. Job,Aldo E. Ferreira,Luciana G. Noseda,Miguel D. Duarte,Maria E. R. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Alexandre,Samara M. A. Silva,Maria de Lourdes Corradi da Vasconcelos,Ana F. D. Cavalcante,Dalita G. S. M. Job,Aldo E. Ferreira,Luciana G. Noseda,Miguel D. Duarte,Maria E. R. |
dc.subject.por.fl_str_mv |
heteropolysaccharide HPSEC/RID HSQC MTT assay |
topic |
heteropolysaccharide HPSEC/RID HSQC MTT assay |
description |
Colletotrichum gloeosporioides biomass was submitted to ethanolic extraction (78 ºC, 12 h) to remove low molecular weight compounds followed by hot aqueous extraction (100 ºC, 4 h, 4x) to obtain polysaccharides. Consecutive freezing/thawing treatments of the aqueous extract yielded a soluble fraction in cold water that was separated on five components (PI to PV) by gel exclusion chromatography on Sepharose CL-6B. The PIV was homogeneous on HPSEC/RID, with apparent MW of 2.8 x 104 g mol-1, Mw/Mn 1.17, 100% of total sugar. The PIV was composed by galactose (61%), glucose (34%) and mannose (5%). 1H and 13C NMR analysis mono- and two-dimensional, homo- (COSY) and heteronuclear (HSQC and HMBC) showed that PIV is a heteropolysacharide with a main chain constituted by β-D-galactofuranosidic units (1→5) and (1→6) linked with some of the β-D-galactofuranosidic (1→6) units substituted in O-3 by α-D-glucopiranosidic residues. Cytotoxic assays (MTT) indicated that the viability of CHO-K1 cells, when exposed to different heteropolysaccharide concentrations, did not show significant differences when compared to the viability of the negative control (CN), in the experimental times of 24 and 48 hours. These results suggest the possibility of new cytotoxic assays in other normal cell lines as well as in tumor cells. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019000400405 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019000400405 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170347 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.42 n.4 2019 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318119649280000 |