Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

Detalhes bibliográficos
Autor(a) principal: Sugawara,Eduardo Kinio
Data de Publicação: 2010
Outros Autores: Ribeiro Neto,Luciane Maria, Verreschi,Ieda Terezinha do Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200036
Resumo: Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
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spelling Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatographyHPLCcortisolurineDetermination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200036Química Nova v.33 n.2 2010reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422010000200036info:eu-repo/semantics/openAccessSugawara,Eduardo KinioRibeiro Neto,Luciane MariaVerreschi,Ieda Terezinha do Nascimentoeng2010-03-12T00:00:00Zoai:scielo:S0100-40422010000200036Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2010-03-12T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
title Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
spellingShingle Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
Sugawara,Eduardo Kinio
HPLC
cortisol
urine
title_short Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
title_full Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
title_fullStr Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
title_full_unstemmed Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
title_sort Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
author Sugawara,Eduardo Kinio
author_facet Sugawara,Eduardo Kinio
Ribeiro Neto,Luciane Maria
Verreschi,Ieda Terezinha do Nascimento
author_role author
author2 Ribeiro Neto,Luciane Maria
Verreschi,Ieda Terezinha do Nascimento
author2_role author
author
dc.contributor.author.fl_str_mv Sugawara,Eduardo Kinio
Ribeiro Neto,Luciane Maria
Verreschi,Ieda Terezinha do Nascimento
dc.subject.por.fl_str_mv HPLC
cortisol
urine
topic HPLC
cortisol
urine
description Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200036
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422010000200036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.33 n.2 2010
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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