Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography

Detalhes bibliográficos
Autor(a) principal: Bispo, Márcia Sousa
Data de Publicação: 2005
Outros Autores: Ferreira, Sergio Luis Costa, Andrade, Jailson Bittencourt de, Aragão, Nádia Machado de, Veloso, Márcia Cristina da Cunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13541
Resumo: Texto completo: acesso restrito. p.1007–1013
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spelling Bispo, Márcia SousaFerreira, Sergio Luis CostaAndrade, Jailson Bittencourt deAragão, Nádia Machado deVeloso, Márcia Cristina da CunhaBispo, Márcia SousaFerreira, Sergio Luis CostaAndrade, Jailson Bittencourt deAragão, Nádia Machado deVeloso, Márcia Cristina da Cunha2013-11-04T11:04:24Z20050039-9140http://repositorio.ufba.br/ri/handle/ri/13541v.67 n. 5Texto completo: acesso restrito. p.1007–1013Caffeine (1,3,7-trimethylxanthine), theobromine (3,7-dimethylxanthine) and theophylline (1,3-dimethylxanthine) are the most important naturally occurring methylxanthines. Caffeine is a constituent of coffee and other beverage and included in many medicines. Theobromine and theophylline are formed as metabolites of caffeine in humans, and are also present in tea, cocoa and chocolate products. In order to improve the chromatographic resolution (Rs) with a good analysis time, experimental designs were applied for multivariate optimisation of the experimental conditions of an isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) method used for the simultaneous determination of caffeine, theobromine and theophylline. The optimisation process was carried out in two steps using full three-level factorial designs. The factors optimised were: flow rate and mobile phase composition. Optimal conditions for the separation of the three methylxanthines were obtained using a mixture of water/ethanol/acetic acid (75:24:1%, v/v/v) as mobile phase and a flow rate of 1.0 mL min−1. The RP-HPLC/UV method was validated in terms of limit of detection (LOD), limit of quantitation (LOQ), linearity, recovery and the precision, calculated as relative standard deviation (R.S.D.). In these conditions, the LOD was 0.10 μg L−1 for caffeine, 0.07 μg L−1 for theobromine and 0.06 μg L−1 for theophylline. The proposed method is fast, requires no extraction step or derivatization and was suitable for quantification of these methylxanthines in coffee, tea and human urine samples.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-11-04T11:04:23Z No. of bitstreams: 1 Márcia Bispo.pdf: 452730 bytes, checksum: ad59f0476c0193172f19b0b9218faef6 (MD5)Made available in DSpace on 2013-11-04T11:04:24Z (GMT). No. of bitstreams: 1 Márcia Bispo.pdf: 452730 bytes, checksum: ad59f0476c0193172f19b0b9218faef6 (MD5) Previous issue date: 2005http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.talanta.2005.04.066reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAMethylxanthinesMultivariate optimisationIsocratic elutionRP-HPLC/UVMultivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatographyTalantainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALMárcia Bispo.pdfMárcia Bispo.pdfapplication/pdf452730https://repositorio.ufba.br/bitstream/ri/13541/1/M%c3%a1rcia%20Bispo.pdfad59f0476c0193172f19b0b9218faef6MD51LICENSElicense.txtlicense.txttext/plain1383https://repositorio.ufba.br/bitstream/ri/13541/2/license.txt05eca2f01d0b3307819d0369dab18a34MD52TEXTMárcia Bispo.pdf.txtMárcia Bispo.pdf.txtExtracted texttext/plain25269https://repositorio.ufba.br/bitstream/ri/13541/3/M%c3%a1rcia%20Bispo.pdf.txt82313913e3b21783f4bc57856aabd65bMD53ri/135412022-07-05 14:03:07.629oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:07Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
dc.title.alternative.pt_BR.fl_str_mv Talanta
title Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
spellingShingle Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
Bispo, Márcia Sousa
Methylxanthines
Multivariate optimisation
Isocratic elution
RP-HPLC/UV
title_short Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
title_full Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
title_fullStr Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
title_full_unstemmed Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
title_sort Multivariate optimisation of the experimental conditions for determination of three methylxanthines by reversed-phase high-performance liquid chromatography
author Bispo, Márcia Sousa
author_facet Bispo, Márcia Sousa
Ferreira, Sergio Luis Costa
Andrade, Jailson Bittencourt de
Aragão, Nádia Machado de
Veloso, Márcia Cristina da Cunha
author_role author
author2 Ferreira, Sergio Luis Costa
Andrade, Jailson Bittencourt de
Aragão, Nádia Machado de
Veloso, Márcia Cristina da Cunha
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bispo, Márcia Sousa
Ferreira, Sergio Luis Costa
Andrade, Jailson Bittencourt de
Aragão, Nádia Machado de
Veloso, Márcia Cristina da Cunha
Bispo, Márcia Sousa
Ferreira, Sergio Luis Costa
Andrade, Jailson Bittencourt de
Aragão, Nádia Machado de
Veloso, Márcia Cristina da Cunha
dc.subject.por.fl_str_mv Methylxanthines
Multivariate optimisation
Isocratic elution
RP-HPLC/UV
topic Methylxanthines
Multivariate optimisation
Isocratic elution
RP-HPLC/UV
description Texto completo: acesso restrito. p.1007–1013
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2013-11-04T11:04:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13541
dc.identifier.issn.none.fl_str_mv 0039-9140
dc.identifier.number.pt_BR.fl_str_mv v.67 n. 5
identifier_str_mv 0039-9140
v.67 n. 5
url http://repositorio.ufba.br/ri/handle/ri/13541
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.talanta.2005.04.066
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instacron_str UFBA
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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