Development and validation of a High Performance Liquid Chromatographic method for determination of etoposide in biodegradable polymeric implants

• Autor(a) principal: Solano,Ana Gabriela Reis
• Data de Publicação: 2012
• Outros Autores: Silva,Gisele Rodrigues da, Fialho,Silvia Ligório, Cunha Júnior,Armando da Silva, Pianetti,Gérson Antônio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000600032
• topic: validation; etoposide; poly(ε-caprolactone); intraocular implant

A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 µm), at 25 ºC, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r² &gt; 0.99) over the range of 5 to 65 µg/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 µg/mL. The validated method can be successfully employed for routine quality control analyses.