Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants

• Autor(a) principal: Saliba,Juliana Barbosa
• Data de Publicação: 2011
• Outros Autores: Cunha Junior,Armando da Silva, Gomes,Elionai Cassiana de Lima, Mansur,Herman Sander, Silva,Gisele Rodrigues da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100026
• topic: cyclosporine-A; biodegradable intraocular implant; validation

An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%.