DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901038 |
Resumo: | A bioanalytical method for HIV detection based on surface-enhanced Raman scattering (SERS) is described. The assay targeted the peptide p17-1. This peptide sequence is present in the protein p17, a structural protein essential in the life cycle of the human immunodeficiency retrovirus (HIV). The antibody anti-p17 was immobilized in a planar gold surface using self-assembled techniques. This planar platform was used to capture the target analyte (p17-1 peptide). The peptide detection was carried out using specially designed SERS probes, consisting of gold nanoparticles coated with a Raman reporter molecule (Nile Blue A) and functionalized with anti-p17. This sandwich assay led to the successful detection of the peptide p17-1 at 40.8 ng/mL levels. The sandwich assay described here demonstrated that the SERS technique can rival more common fluorescence methods, such as ELISA, in the detection HIV-type virus. |
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DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS)HIVSERSpeptide p17-1SERS probesSERS Immunoassay.A bioanalytical method for HIV detection based on surface-enhanced Raman scattering (SERS) is described. The assay targeted the peptide p17-1. This peptide sequence is present in the protein p17, a structural protein essential in the life cycle of the human immunodeficiency retrovirus (HIV). The antibody anti-p17 was immobilized in a planar gold surface using self-assembled techniques. This planar platform was used to capture the target analyte (p17-1 peptide). The peptide detection was carried out using specially designed SERS probes, consisting of gold nanoparticles coated with a Raman reporter molecule (Nile Blue A) and functionalized with anti-p17. This sandwich assay led to the successful detection of the peptide p17-1 at 40.8 ng/mL levels. The sandwich assay described here demonstrated that the SERS technique can rival more common fluorescence methods, such as ELISA, in the detection HIV-type virus.Sociedade Brasileira de Química2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901038Química Nova v.42 n.9 2019reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170430info:eu-repo/semantics/openAccessCarneiro,Leandro B.Moraes,Marli L.Ribeiro,Sidney J. L.Brolo,Alexandre G.eng2019-12-09T00:00:00Zoai:scielo:S0100-40422019004901038Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-12-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
title |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
spellingShingle |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) Carneiro,Leandro B. HIV SERS peptide p17-1 SERS probes SERS Immunoassay. |
title_short |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
title_full |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
title_fullStr |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
title_full_unstemmed |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
title_sort |
DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS) |
author |
Carneiro,Leandro B. |
author_facet |
Carneiro,Leandro B. Moraes,Marli L. Ribeiro,Sidney J. L. Brolo,Alexandre G. |
author_role |
author |
author2 |
Moraes,Marli L. Ribeiro,Sidney J. L. Brolo,Alexandre G. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Carneiro,Leandro B. Moraes,Marli L. Ribeiro,Sidney J. L. Brolo,Alexandre G. |
dc.subject.por.fl_str_mv |
HIV SERS peptide p17-1 SERS probes SERS Immunoassay. |
topic |
HIV SERS peptide p17-1 SERS probes SERS Immunoassay. |
description |
A bioanalytical method for HIV detection based on surface-enhanced Raman scattering (SERS) is described. The assay targeted the peptide p17-1. This peptide sequence is present in the protein p17, a structural protein essential in the life cycle of the human immunodeficiency retrovirus (HIV). The antibody anti-p17 was immobilized in a planar gold surface using self-assembled techniques. This planar platform was used to capture the target analyte (p17-1 peptide). The peptide detection was carried out using specially designed SERS probes, consisting of gold nanoparticles coated with a Raman reporter molecule (Nile Blue A) and functionalized with anti-p17. This sandwich assay led to the successful detection of the peptide p17-1 at 40.8 ng/mL levels. The sandwich assay described here demonstrated that the SERS technique can rival more common fluorescence methods, such as ELISA, in the detection HIV-type virus. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901038 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901038 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170430 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.42 n.9 2019 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318120045641728 |