MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020001001385 |
Resumo: | Cyanobacteria produce a wide variety of bioactive compounds, making them a highly promising group in the search for novel molecules with pharmacological and biotechnological properties. Among these, microginins attract attention for being peptides which inhibit angiotensin-converting enzyme, turning them into potential targets for hypertension and congestive heart failure treatment. This work describes a rapid and sensitive method for untarget screening of microginins in cyanobacteria extracts by LC-QqQ-MS/MS. These compounds are mostly characterized by containing 3-amino-2-hydroxy-decanoic acid at the N-terminus, which often could be chlorinated, dichlorinated or methylated. Based on the fragment ion arising from this decanoic acid derivative, a precursor ion scan (PIS) strategy has been proposed. This approach identified suspect microginins in cyanobacterial strains and environmental samples that were later confirmed by LC-QTOF-MS/MS. Eight new microginins structures were characterized based on the obtained fragmentation spectra from a total of 19 variants detected. This study highlights the applicability of PIS mode acquisition for untarget screening, detecting a wide variety of microginins with amino acids modifications, produced mainly by Microcystis aeruginosa strains. This method is a useful tool for the identification and environmental monitoring of molecules with conserved molecular substructures that possess similar fragmentation pattern |
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MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MScyanobacteriacyanopeptidesmicrogininprecursor ion scanLC-MS/MSCyanobacteria produce a wide variety of bioactive compounds, making them a highly promising group in the search for novel molecules with pharmacological and biotechnological properties. Among these, microginins attract attention for being peptides which inhibit angiotensin-converting enzyme, turning them into potential targets for hypertension and congestive heart failure treatment. This work describes a rapid and sensitive method for untarget screening of microginins in cyanobacteria extracts by LC-QqQ-MS/MS. These compounds are mostly characterized by containing 3-amino-2-hydroxy-decanoic acid at the N-terminus, which often could be chlorinated, dichlorinated or methylated. Based on the fragment ion arising from this decanoic acid derivative, a precursor ion scan (PIS) strategy has been proposed. This approach identified suspect microginins in cyanobacterial strains and environmental samples that were later confirmed by LC-QTOF-MS/MS. Eight new microginins structures were characterized based on the obtained fragmentation spectra from a total of 19 variants detected. This study highlights the applicability of PIS mode acquisition for untarget screening, detecting a wide variety of microginins with amino acids modifications, produced mainly by Microcystis aeruginosa strains. This method is a useful tool for the identification and environmental monitoring of molecules with conserved molecular substructures that possess similar fragmentation patternSociedade Brasileira de Química2020-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020001001385Química Nova v.43 n.10 2020reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170640info:eu-repo/semantics/openAccessTeramoto,Kazumi K.Dorr,FabianeSanz,MiriamPinto,Ernanipor2021-02-11T00:00:00Zoai:scielo:S0100-40422020001001385Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2021-02-11T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
title |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
spellingShingle |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS Teramoto,Kazumi K. cyanobacteria cyanopeptides microginin precursor ion scan LC-MS/MS |
title_short |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
title_full |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
title_fullStr |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
title_full_unstemmed |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
title_sort |
MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS |
author |
Teramoto,Kazumi K. |
author_facet |
Teramoto,Kazumi K. Dorr,Fabiane Sanz,Miriam Pinto,Ernani |
author_role |
author |
author2 |
Dorr,Fabiane Sanz,Miriam Pinto,Ernani |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Teramoto,Kazumi K. Dorr,Fabiane Sanz,Miriam Pinto,Ernani |
dc.subject.por.fl_str_mv |
cyanobacteria cyanopeptides microginin precursor ion scan LC-MS/MS |
topic |
cyanobacteria cyanopeptides microginin precursor ion scan LC-MS/MS |
description |
Cyanobacteria produce a wide variety of bioactive compounds, making them a highly promising group in the search for novel molecules with pharmacological and biotechnological properties. Among these, microginins attract attention for being peptides which inhibit angiotensin-converting enzyme, turning them into potential targets for hypertension and congestive heart failure treatment. This work describes a rapid and sensitive method for untarget screening of microginins in cyanobacteria extracts by LC-QqQ-MS/MS. These compounds are mostly characterized by containing 3-amino-2-hydroxy-decanoic acid at the N-terminus, which often could be chlorinated, dichlorinated or methylated. Based on the fragment ion arising from this decanoic acid derivative, a precursor ion scan (PIS) strategy has been proposed. This approach identified suspect microginins in cyanobacterial strains and environmental samples that were later confirmed by LC-QTOF-MS/MS. Eight new microginins structures were characterized based on the obtained fragmentation spectra from a total of 19 variants detected. This study highlights the applicability of PIS mode acquisition for untarget screening, detecting a wide variety of microginins with amino acids modifications, produced mainly by Microcystis aeruginosa strains. This method is a useful tool for the identification and environmental monitoring of molecules with conserved molecular substructures that possess similar fragmentation pattern |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020001001385 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020001001385 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170640 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.43 n.10 2020 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318120667447296 |