Investigação eletroquímica e calorimétrica da interação de novos agentes antitumorais biscatiônicos com DNA

• Autor(a) principal: Silva,Láuris Lucia da
• Data de Publicação: 2012
• Outros Autores: Donnici,Claudio Luis, Lopes,Júlio César Dias, Goulart,Marília Oliveira Fonseca, Abreu,Fabiane Caxico de, Paula,Francine Santos de, Bravo,Carlos E. Salas, Santoro,Marcelo Matos, Denadai,Ângelo Márcio Leite, Santos,Alexandre Martins Costa, Montanari,Carlos Alberto
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000700007
• topic: biscationic compounds and interaction with DNA; isothermal titration calorimetry; electrochemical biosensor

Biscationic amidines bind in the DNA minor groove and present biological activity against a range of infectious diseases. Two new biscationic compounds (bis-α,ω-S-thioureido, amino and sulfide analogues) were synthesized in good yields and fully characterized, and their interaction with DNA was also investigated. Isothermal titration calorimetry (ITC) was used to measure the thermodynamic properties of binding interactions between DNA and these ligands. A double stranded calf thymus DNA immobilized on an electrode surface was used to study the possible DNA-interacting abilities of these compounds towards dsDNA in situ. A remarkable interaction of these compounds with DNA was demonstrated and their potential application as anticancer agents was furthered.