Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Química Nova (Online) |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100023 |
Resumo: | A liquid chromatography method was developed and validated for the determination of phenobarbital in human plasma using phenytoin as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C12 analytical column, maintained at 35 ºC, with water:acetonitrile:methanol (58.8:15.2:26, v/v/v) as mobile phase, run at a flow rate of 1.2 mL/min with detection at 205 nm. The method was linear in the range of 0.1-4 μg/mL (r²=0.9999) and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations (test and reference) of phenobarbital 100 mg after single oral dose administration to healthy human volunteers. |
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Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence studyhuman plasmaliquid chromatographyphenobarbitalA liquid chromatography method was developed and validated for the determination of phenobarbital in human plasma using phenytoin as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C12 analytical column, maintained at 35 ºC, with water:acetonitrile:methanol (58.8:15.2:26, v/v/v) as mobile phase, run at a flow rate of 1.2 mL/min with detection at 205 nm. The method was linear in the range of 0.1-4 μg/mL (r²=0.9999) and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations (test and reference) of phenobarbital 100 mg after single oral dose administration to healthy human volunteers.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100023Química Nova v.33 n.1 2010reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422010000100023info:eu-repo/semantics/openAccessDalmora,Sérgio LuizSangoi,Maximiliano da SilvaNogueira,Daniele RubertD'Avila,Felipe BianchiniMoreno,Ronílson AgnaldoSverdloff,Carlos EduardoOliveira,Rogério Antonio deBorges,Ney Cartereng2010-02-05T00:00:00Zoai:scielo:S0100-40422010000100023Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2010-02-05T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
| dc.title.none.fl_str_mv |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| title |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| spellingShingle |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study Dalmora,Sérgio Luiz human plasma liquid chromatography phenobarbital |
| title_short |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| title_full |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| title_fullStr |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| title_full_unstemmed |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| title_sort |
Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study |
| author |
Dalmora,Sérgio Luiz |
| author_facet |
Dalmora,Sérgio Luiz Sangoi,Maximiliano da Silva Nogueira,Daniele Rubert D'Avila,Felipe Bianchini Moreno,Ronílson Agnaldo Sverdloff,Carlos Eduardo Oliveira,Rogério Antonio de Borges,Ney Carter |
| author_role |
author |
| author2 |
Sangoi,Maximiliano da Silva Nogueira,Daniele Rubert D'Avila,Felipe Bianchini Moreno,Ronílson Agnaldo Sverdloff,Carlos Eduardo Oliveira,Rogério Antonio de Borges,Ney Carter |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Dalmora,Sérgio Luiz Sangoi,Maximiliano da Silva Nogueira,Daniele Rubert D'Avila,Felipe Bianchini Moreno,Ronílson Agnaldo Sverdloff,Carlos Eduardo Oliveira,Rogério Antonio de Borges,Ney Carter |
| dc.subject.por.fl_str_mv |
human plasma liquid chromatography phenobarbital |
| topic |
human plasma liquid chromatography phenobarbital |
| description |
A liquid chromatography method was developed and validated for the determination of phenobarbital in human plasma using phenytoin as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C12 analytical column, maintained at 35 ºC, with water:acetonitrile:methanol (58.8:15.2:26, v/v/v) as mobile phase, run at a flow rate of 1.2 mL/min with detection at 205 nm. The method was linear in the range of 0.1-4 μg/mL (r²=0.9999) and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations (test and reference) of phenobarbital 100 mg after single oral dose administration to healthy human volunteers. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-01-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100023 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000100023 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S0100-40422010000100023 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
| dc.source.none.fl_str_mv |
Química Nova v.33 n.1 2010 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| instname_str |
Sociedade Brasileira de Química (SBQ) |
| instacron_str |
SBQ |
| institution |
SBQ |
| reponame_str |
Química Nova (Online) |
| collection |
Química Nova (Online) |
| repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
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quimicanova@sbq.org.br |
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1750318109979312128 |