Determination of flutamide in tablets by high-performance liquid chromatography

• Autor(a) principal: Salgado, Hérida Regina Nunes [UNESP]
• Data de Publicação: 2005
• Outros Autores: De Menezes, Manoel [UNESP], Storti, Madge Patrícia Betto [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.latamjpharm.org/trabajos/24/2/LAJOP_24_2_3_1_48439399MF.pdf; http://hdl.handle.net/11449/68197
• topic: Analysis in tablets; Flutamide; High-performance liquid chromatography (HPLC); Pharmaceutical analysis; acetonitrile; flutamide; drug determination; high performance liquid chromatography; prostate cancer; reversed phase high performance liquid chromatography; tablet

Flutamide is a potent antiandrogen used for the treatment of prostatic cancer. A simple, sensitive and accurate high-performance liquid Chromatographic (HPLC) method is presented for quantitative determination of flutamide in tablets, using a reversed-phase technique and UV detection at 240 nm. The isocratic elution was used to quantify the analyte. The samples were chromatographed on Luna-C18 column and the mobile phase was 0.05 M phosphate buffer pH 4.0 - acetonitrile (50:50, v/v). The method was linear between 2.9 - 11.6 mg L -1. Over the tested concentration range the intra-day relative standard deviation for replicate analysis in tablets ranged from 0.44 to 0.78%. It was also found that the excipients in the commercial tablets did not interfere with the method.