BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000500572 |
Resumo: | Production of bacterial nanocellulose was pursued as a matrix system for the stabilization of human insulin. The biomembranes produced by Gluconacetobacter hansenii were washed with 2% aqueous sodium dodecylsulfate solution, rinsed with ultrapure water and immersed in 1 mol L-1 NaOH aqueous solution at 60 °C for 90 min until neutralization. For the insulin adsorption assays, the biomembranes were soaked in a buffered solution of human insulin until no protein could be detected in the supernatant. The membranes with adsorbed insulin were characterized via mechanical resistance (resilience, relaxation, perforation), Differential Scanning Calorimetry (DSC), Thermal Gravimetrical Analysis (TGA), Fourier Transform Infrared Spectrophotometry (FTIR), X-ray diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analyses. The FESEM photomicrographs of the surface of the biomembranes showed a rugged surface without cracks. The biomembranes exhibited adequate mechanical characteristics. The infrared spectra indicated that the chemical aspect of the protein moiety was preserved during adsorption onto the BNC biomembranes. According to the XRD analyses, the biomembranes showed a generalized amorphous behavior. Thermal analyses indicated an adequate thermal stability for a pharmaceuticals product. Hence, an elastic and malleable biomembrane was produced, suitable for incorporation of human insulin, aiming at transdermal delivery. |
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BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATIONrecombinant human insulinnanocellulose biomembranesexopolysaccharyde productiontransdermal permeationProduction of bacterial nanocellulose was pursued as a matrix system for the stabilization of human insulin. The biomembranes produced by Gluconacetobacter hansenii were washed with 2% aqueous sodium dodecylsulfate solution, rinsed with ultrapure water and immersed in 1 mol L-1 NaOH aqueous solution at 60 °C for 90 min until neutralization. For the insulin adsorption assays, the biomembranes were soaked in a buffered solution of human insulin until no protein could be detected in the supernatant. The membranes with adsorbed insulin were characterized via mechanical resistance (resilience, relaxation, perforation), Differential Scanning Calorimetry (DSC), Thermal Gravimetrical Analysis (TGA), Fourier Transform Infrared Spectrophotometry (FTIR), X-ray diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analyses. The FESEM photomicrographs of the surface of the biomembranes showed a rugged surface without cracks. The biomembranes exhibited adequate mechanical characteristics. The infrared spectra indicated that the chemical aspect of the protein moiety was preserved during adsorption onto the BNC biomembranes. According to the XRD analyses, the biomembranes showed a generalized amorphous behavior. Thermal analyses indicated an adequate thermal stability for a pharmaceuticals product. Hence, an elastic and malleable biomembrane was produced, suitable for incorporation of human insulin, aiming at transdermal delivery.Sociedade Brasileira de Química2020-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000500572Química Nova v.43 n.5 2020reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170522info:eu-repo/semantics/openAccessJorge,Ludmilla R.Harada,Liliam K.Silva,Erica C.Campos,Welida F.Oliveira Jr.,José M.Vila,Marta M. D. C.Tubino,MatthieuBalcão,Victor M.eng2020-06-25T00:00:00Zoai:scielo:S0100-40422020000500572Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2020-06-25T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
title |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
spellingShingle |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION Jorge,Ludmilla R. recombinant human insulin nanocellulose biomembranes exopolysaccharyde production transdermal permeation |
title_short |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
title_full |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
title_fullStr |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
title_full_unstemmed |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
title_sort |
BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION |
author |
Jorge,Ludmilla R. |
author_facet |
Jorge,Ludmilla R. Harada,Liliam K. Silva,Erica C. Campos,Welida F. Oliveira Jr.,José M. Vila,Marta M. D. C. Tubino,Matthieu Balcão,Victor M. |
author_role |
author |
author2 |
Harada,Liliam K. Silva,Erica C. Campos,Welida F. Oliveira Jr.,José M. Vila,Marta M. D. C. Tubino,Matthieu Balcão,Victor M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Jorge,Ludmilla R. Harada,Liliam K. Silva,Erica C. Campos,Welida F. Oliveira Jr.,José M. Vila,Marta M. D. C. Tubino,Matthieu Balcão,Victor M. |
dc.subject.por.fl_str_mv |
recombinant human insulin nanocellulose biomembranes exopolysaccharyde production transdermal permeation |
topic |
recombinant human insulin nanocellulose biomembranes exopolysaccharyde production transdermal permeation |
description |
Production of bacterial nanocellulose was pursued as a matrix system for the stabilization of human insulin. The biomembranes produced by Gluconacetobacter hansenii were washed with 2% aqueous sodium dodecylsulfate solution, rinsed with ultrapure water and immersed in 1 mol L-1 NaOH aqueous solution at 60 °C for 90 min until neutralization. For the insulin adsorption assays, the biomembranes were soaked in a buffered solution of human insulin until no protein could be detected in the supernatant. The membranes with adsorbed insulin were characterized via mechanical resistance (resilience, relaxation, perforation), Differential Scanning Calorimetry (DSC), Thermal Gravimetrical Analysis (TGA), Fourier Transform Infrared Spectrophotometry (FTIR), X-ray diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analyses. The FESEM photomicrographs of the surface of the biomembranes showed a rugged surface without cracks. The biomembranes exhibited adequate mechanical characteristics. The infrared spectra indicated that the chemical aspect of the protein moiety was preserved during adsorption onto the BNC biomembranes. According to the XRD analyses, the biomembranes showed a generalized amorphous behavior. Thermal analyses indicated an adequate thermal stability for a pharmaceuticals product. Hence, an elastic and malleable biomembrane was produced, suitable for incorporation of human insulin, aiming at transdermal delivery. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000500572 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000500572 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170522 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.43 n.5 2020 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318120484995072 |