DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS

Detalhes bibliográficos
Autor(a) principal: Coura,Júlia Cecília Pereira
Data de Publicação: 2021
Outros Autores: Silva,Cláudia Aparecida de Oliveira e, Silva,Edvane Santos, Valladão,Sara Araújo, Duarte,Maria Gorette Resende
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422021001001353
Resumo: Caffeine and guarana are safe foods according to the American (FDA) and Brazilian (ANVISA) health agencies. However, data regarding the composition, quality, and safety of guarana-based food supplements sold in Brazil are limited. Most of the methods used for quantification of caffeine and other guarana chemical markers are based on complex extraction techniques as well as on gradient elution and do not evaluate the matrix effect nor the uncertainty estimation measurement. A simple and selective method for caffeine detection has been developed and validated using HPLC-UV. It shows linearity between 1 and 10 µg mL-1, has a significant matrix effect (p < 0.05) and its expanded uncertainty varies from 6.9 to 16.7%. Other parameters (selectivity, recovery, precision, robustness, limits of detection, and quantification) were satisfactory. The present study has analyzed 30 commercial samples of guarana-based food supplements (powders and capsules). Powder samples have shown an average caffeine level of 25.27 ± 5.20 mg g-1 while capsules 28.53 ± 13.81 mg g-1. No significant difference between the two types of samples has been observed (p > 0.005).
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spelling DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTSbioactive substancesfood supplementshigh performance liquid chromatographymethylxanthinesquality controlCaffeine and guarana are safe foods according to the American (FDA) and Brazilian (ANVISA) health agencies. However, data regarding the composition, quality, and safety of guarana-based food supplements sold in Brazil are limited. Most of the methods used for quantification of caffeine and other guarana chemical markers are based on complex extraction techniques as well as on gradient elution and do not evaluate the matrix effect nor the uncertainty estimation measurement. A simple and selective method for caffeine detection has been developed and validated using HPLC-UV. It shows linearity between 1 and 10 µg mL-1, has a significant matrix effect (p < 0.05) and its expanded uncertainty varies from 6.9 to 16.7%. Other parameters (selectivity, recovery, precision, robustness, limits of detection, and quantification) were satisfactory. The present study has analyzed 30 commercial samples of guarana-based food supplements (powders and capsules). Powder samples have shown an average caffeine level of 25.27 ± 5.20 mg g-1 while capsules 28.53 ± 13.81 mg g-1. No significant difference between the two types of samples has been observed (p > 0.005).Sociedade Brasileira de Química2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422021001001353Química Nova v.44 n.10 2021reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170774info:eu-repo/semantics/openAccessCoura,Júlia Cecília PereiraSilva,Cláudia Aparecida de Oliveira eSilva,Edvane SantosValladão,Sara AraújoDuarte,Maria Gorette Resendeeng2021-12-16T00:00:00Zoai:scielo:S0100-40422021001001353Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2021-12-16T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
title DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
spellingShingle DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
Coura,Júlia Cecília Pereira
bioactive substances
food supplements
high performance liquid chromatography
methylxanthines
quality control
title_short DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
title_full DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
title_fullStr DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
title_full_unstemmed DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
title_sort DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS
author Coura,Júlia Cecília Pereira
author_facet Coura,Júlia Cecília Pereira
Silva,Cláudia Aparecida de Oliveira e
Silva,Edvane Santos
Valladão,Sara Araújo
Duarte,Maria Gorette Resende
author_role author
author2 Silva,Cláudia Aparecida de Oliveira e
Silva,Edvane Santos
Valladão,Sara Araújo
Duarte,Maria Gorette Resende
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Coura,Júlia Cecília Pereira
Silva,Cláudia Aparecida de Oliveira e
Silva,Edvane Santos
Valladão,Sara Araújo
Duarte,Maria Gorette Resende
dc.subject.por.fl_str_mv bioactive substances
food supplements
high performance liquid chromatography
methylxanthines
quality control
topic bioactive substances
food supplements
high performance liquid chromatography
methylxanthines
quality control
description Caffeine and guarana are safe foods according to the American (FDA) and Brazilian (ANVISA) health agencies. However, data regarding the composition, quality, and safety of guarana-based food supplements sold in Brazil are limited. Most of the methods used for quantification of caffeine and other guarana chemical markers are based on complex extraction techniques as well as on gradient elution and do not evaluate the matrix effect nor the uncertainty estimation measurement. A simple and selective method for caffeine detection has been developed and validated using HPLC-UV. It shows linearity between 1 and 10 µg mL-1, has a significant matrix effect (p < 0.05) and its expanded uncertainty varies from 6.9 to 16.7%. Other parameters (selectivity, recovery, precision, robustness, limits of detection, and quantification) were satisfactory. The present study has analyzed 30 commercial samples of guarana-based food supplements (powders and capsules). Powder samples have shown an average caffeine level of 25.27 ± 5.20 mg g-1 while capsules 28.53 ± 13.81 mg g-1. No significant difference between the two types of samples has been observed (p > 0.005).
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422021001001353
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422021001001353
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0100-4042.20170774
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.44 n.10 2021
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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