Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/212197 |
Resumo: | Guarana (Paullinia cupana) is a native plant from the Amazon whose seeds contain a high concentration of caffeine. Aqueous extract of guarana is widely used in the world. In this study, the objective was to develop and validate a High-Performance Liquid Chromatography method for the determination of caffeine in extracts and commercial beverages based on guarana. A sensitive, simple, and viable high performance liquid chromatographic method without the need of an analyte extraction procedure was developed and validated according to Brazilian and international requirements. The method presented high performance, fulfilling Brazilian and international requirements, in addition to allowing product compliance tests. Results confirmed high selectivity and linearity (>0.999) between 5 to 135 ug/mL, with no significant matrix effect. Detection and quantification limits were 0.02 µg/mL and 2 µg/mL, respectively. Precision was less than 4 %, and accuracy varied from 99.9-120 %. Applicability of the method was demonstrated by conducting a limited evaluation in products containing caffeine. Commercial extracts showed quite different caffeine levels, while carbonated drinks follow Brazilian and American recommendations. Our results indicate that the developed method can be used to evaluate the quality of the guarana extract and of products containing caffeine. |
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Brazilian Journal of Pharmaceutical Sciences |
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Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana)Paullinia cupanaCaffeine; Guarana extractHigh performance liquid chromatographyGuarana (Paullinia cupana) is a native plant from the Amazon whose seeds contain a high concentration of caffeine. Aqueous extract of guarana is widely used in the world. In this study, the objective was to develop and validate a High-Performance Liquid Chromatography method for the determination of caffeine in extracts and commercial beverages based on guarana. A sensitive, simple, and viable high performance liquid chromatographic method without the need of an analyte extraction procedure was developed and validated according to Brazilian and international requirements. The method presented high performance, fulfilling Brazilian and international requirements, in addition to allowing product compliance tests. Results confirmed high selectivity and linearity (>0.999) between 5 to 135 ug/mL, with no significant matrix effect. Detection and quantification limits were 0.02 µg/mL and 2 µg/mL, respectively. Precision was less than 4 %, and accuracy varied from 99.9-120 %. Applicability of the method was demonstrated by conducting a limited evaluation in products containing caffeine. Commercial extracts showed quite different caffeine levels, while carbonated drinks follow Brazilian and American recommendations. Our results indicate that the developed method can be used to evaluate the quality of the guarana extract and of products containing caffeine.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2023-05-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/21219710.1590/s2175-97902023e22106Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023); https://doi.org/10.1590/s2175-97902023e22106Brazilian Journal of Pharmaceutical Sciences; v. 59 (2023); https://doi.org/10.1590/s2175-97902023e22106Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023); https://doi.org/10.1590/s2175-97902023e221062175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/212197/194313https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMoura-Silva, Anna KellyGonçalves, Daniel BonotoMano-Sousa, Brayan JonasSantos, Lays Pedrosada Costa, Rafael SerrãoAndrade, FrankDuarte-Almeida, Joaquim M.2023-05-24T17:38:29Zoai:revistas.usp.br:article/212197Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-05-24T17:38:29Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
title |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
spellingShingle |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) Moura-Silva, Anna Kelly Paullinia cupana Caffeine; Guarana extract High performance liquid chromatography |
title_short |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
title_full |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
title_fullStr |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
title_full_unstemmed |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
title_sort |
Development and validation of an analytical method by HPLC-DAD for determination of caffeine in products based on guarana extracts (Paullinia cupana) |
author |
Moura-Silva, Anna Kelly |
author_facet |
Moura-Silva, Anna Kelly Gonçalves, Daniel Bonoto Mano-Sousa, Brayan Jonas Santos, Lays Pedrosa da Costa, Rafael Serrão Andrade, Frank Duarte-Almeida, Joaquim M. |
author_role |
author |
author2 |
Gonçalves, Daniel Bonoto Mano-Sousa, Brayan Jonas Santos, Lays Pedrosa da Costa, Rafael Serrão Andrade, Frank Duarte-Almeida, Joaquim M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Moura-Silva, Anna Kelly Gonçalves, Daniel Bonoto Mano-Sousa, Brayan Jonas Santos, Lays Pedrosa da Costa, Rafael Serrão Andrade, Frank Duarte-Almeida, Joaquim M. |
dc.subject.por.fl_str_mv |
Paullinia cupana Caffeine; Guarana extract High performance liquid chromatography |
topic |
Paullinia cupana Caffeine; Guarana extract High performance liquid chromatography |
description |
Guarana (Paullinia cupana) is a native plant from the Amazon whose seeds contain a high concentration of caffeine. Aqueous extract of guarana is widely used in the world. In this study, the objective was to develop and validate a High-Performance Liquid Chromatography method for the determination of caffeine in extracts and commercial beverages based on guarana. A sensitive, simple, and viable high performance liquid chromatographic method without the need of an analyte extraction procedure was developed and validated according to Brazilian and international requirements. The method presented high performance, fulfilling Brazilian and international requirements, in addition to allowing product compliance tests. Results confirmed high selectivity and linearity (>0.999) between 5 to 135 ug/mL, with no significant matrix effect. Detection and quantification limits were 0.02 µg/mL and 2 µg/mL, respectively. Precision was less than 4 %, and accuracy varied from 99.9-120 %. Applicability of the method was demonstrated by conducting a limited evaluation in products containing caffeine. Commercial extracts showed quite different caffeine levels, while carbonated drinks follow Brazilian and American recommendations. Our results indicate that the developed method can be used to evaluate the quality of the guarana extract and of products containing caffeine. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/212197 10.1590/s2175-97902023e22106 |
url |
https://www.revistas.usp.br/bjps/article/view/212197 |
identifier_str_mv |
10.1590/s2175-97902023e22106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/212197/194313 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023); https://doi.org/10.1590/s2175-97902023e22106 Brazilian Journal of Pharmaceutical Sciences; v. 59 (2023); https://doi.org/10.1590/s2175-97902023e22106 Brazilian Journal of Pharmaceutical Sciences; Vol. 59 (2023); https://doi.org/10.1590/s2175-97902023e22106 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222918096977920 |