CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200181 |
Resumo: | Phytochemical study of aerial parts from Sinningia mauroana Chautems (Gesneriaceae) yielded 17 known compounds: sitosterol, stigmasterol, betulinic acid, oleanolic acid, hederagenin, maslinic acid, ursolic acid, pomolic acid, soranjidiol, methyl 4-hydroxyphenylacetate, 6-hydroxy-7-methoxy-α-dunnione, 7-hydroxy-6-methoxy-α-dunnione, jacaranone, calceolarioside A, conandroside, luteolin, and luteolin-7-O-glucoside. Complete NMR data of 6-hydroxy-7-methoxy-α-dunnione and 7-hydroxy-6-methoxy-α-dunnione were obtained in two different solvents. Hexane, ethyl acetate and ethanol extracts were screened for antimicrobial, antioxidant and cytotoxic activities. The antimicrobial activity was assayed against Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, C. parapsilosis, C. dubliniensis, and C. glabrata, using the microdillution method. All extracts were inactive (MIC > 100 µg mL-1). The antioxidant activity was evaluated with the ORAC method; the ethyl acetate and ethanol extracts were active (TE relative > 800 µg g-1). The cytotoxic activity was tested against PC-3 (prostate) and SKMEL-103 (melanoma) human tumor cell lines, and 3T3 fibroblast cell line, using the MTT assay. The hexane extract showed strong activity against PC-3 and SKMEL-103 human tumor cell lines (IC50 < 0.25 and 3.07 µg mL-1, respectively), and a lower activity towards 3T3 fibroblasts (IC50 = 6.84 µg mL-1). The other extracts were inactive (IC50 > 50 µg mL-1). |
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CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIESSinningia mauroanaGesneriaceaetriterpenesnaphthoquinonesphenolic compoundsPhytochemical study of aerial parts from Sinningia mauroana Chautems (Gesneriaceae) yielded 17 known compounds: sitosterol, stigmasterol, betulinic acid, oleanolic acid, hederagenin, maslinic acid, ursolic acid, pomolic acid, soranjidiol, methyl 4-hydroxyphenylacetate, 6-hydroxy-7-methoxy-α-dunnione, 7-hydroxy-6-methoxy-α-dunnione, jacaranone, calceolarioside A, conandroside, luteolin, and luteolin-7-O-glucoside. Complete NMR data of 6-hydroxy-7-methoxy-α-dunnione and 7-hydroxy-6-methoxy-α-dunnione were obtained in two different solvents. Hexane, ethyl acetate and ethanol extracts were screened for antimicrobial, antioxidant and cytotoxic activities. The antimicrobial activity was assayed against Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, C. parapsilosis, C. dubliniensis, and C. glabrata, using the microdillution method. All extracts were inactive (MIC > 100 µg mL-1). The antioxidant activity was evaluated with the ORAC method; the ethyl acetate and ethanol extracts were active (TE relative > 800 µg g-1). The cytotoxic activity was tested against PC-3 (prostate) and SKMEL-103 (melanoma) human tumor cell lines, and 3T3 fibroblast cell line, using the MTT assay. The hexane extract showed strong activity against PC-3 and SKMEL-103 human tumor cell lines (IC50 < 0.25 and 3.07 µg mL-1, respectively), and a lower activity towards 3T3 fibroblasts (IC50 = 6.84 µg mL-1). The other extracts were inactive (IC50 > 50 µg mL-1).Sociedade Brasileira de Química2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200181Química Nova v.43 n.2 2020reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170478info:eu-repo/semantics/openAccessWiniewski,VanessaSerain,Alessandra F.Sá,Eduardo L. deSalvador,Marcos J.Stefanello,Maria Élida A.eng2020-05-12T00:00:00Zoai:scielo:S0100-40422020000200181Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2020-05-12T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
title |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
spellingShingle |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES Winiewski,Vanessa Sinningia mauroana Gesneriaceae triterpenes naphthoquinones phenolic compounds |
title_short |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
title_full |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
title_fullStr |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
title_full_unstemmed |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
title_sort |
CHEMICAL CONSTITUENTS OF Sinningia mauroana AND SCREENING OF ITS EXTRACTS FOR ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES |
author |
Winiewski,Vanessa |
author_facet |
Winiewski,Vanessa Serain,Alessandra F. Sá,Eduardo L. de Salvador,Marcos J. Stefanello,Maria Élida A. |
author_role |
author |
author2 |
Serain,Alessandra F. Sá,Eduardo L. de Salvador,Marcos J. Stefanello,Maria Élida A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Winiewski,Vanessa Serain,Alessandra F. Sá,Eduardo L. de Salvador,Marcos J. Stefanello,Maria Élida A. |
dc.subject.por.fl_str_mv |
Sinningia mauroana Gesneriaceae triterpenes naphthoquinones phenolic compounds |
topic |
Sinningia mauroana Gesneriaceae triterpenes naphthoquinones phenolic compounds |
description |
Phytochemical study of aerial parts from Sinningia mauroana Chautems (Gesneriaceae) yielded 17 known compounds: sitosterol, stigmasterol, betulinic acid, oleanolic acid, hederagenin, maslinic acid, ursolic acid, pomolic acid, soranjidiol, methyl 4-hydroxyphenylacetate, 6-hydroxy-7-methoxy-α-dunnione, 7-hydroxy-6-methoxy-α-dunnione, jacaranone, calceolarioside A, conandroside, luteolin, and luteolin-7-O-glucoside. Complete NMR data of 6-hydroxy-7-methoxy-α-dunnione and 7-hydroxy-6-methoxy-α-dunnione were obtained in two different solvents. Hexane, ethyl acetate and ethanol extracts were screened for antimicrobial, antioxidant and cytotoxic activities. The antimicrobial activity was assayed against Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, C. parapsilosis, C. dubliniensis, and C. glabrata, using the microdillution method. All extracts were inactive (MIC > 100 µg mL-1). The antioxidant activity was evaluated with the ORAC method; the ethyl acetate and ethanol extracts were active (TE relative > 800 µg g-1). The cytotoxic activity was tested against PC-3 (prostate) and SKMEL-103 (melanoma) human tumor cell lines, and 3T3 fibroblast cell line, using the MTT assay. The hexane extract showed strong activity against PC-3 and SKMEL-103 human tumor cell lines (IC50 < 0.25 and 3.07 µg mL-1, respectively), and a lower activity towards 3T3 fibroblasts (IC50 = 6.84 µg mL-1). The other extracts were inactive (IC50 > 50 µg mL-1). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200181 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170478 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.43 n.2 2020 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
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1750318120120090624 |