Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada

Detalhes bibliográficos
Autor(a) principal: Ferracini-Santos,Luciana
Data de Publicação: 2009
Outros Autores: Sato,Hélia Harumi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200010
Resumo: Yeast cell wall contains polymers glucan and mannan-protein that have received much attention with respect to their biological activities. Conventional isolation process involving treatments with hot alkali and acids cause degradation of these polymers. The aim of this paper was to study a low-degrading process for the isolation of glucan and mannan-protein from S. cerevisiae cell wall comprising physic and enzymatic treatments. Yeast cell glucan was obtained in a purity of 87.4% and a yield of 33.7%. The isolated mannan-protein presented antioxidant activity that was increased after thirty minutes of protease treatment. Antioxidant activity was determined by β-carotene/linoleate model system.
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spelling Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isoladayeast glucanantioxidant activitymannan-proteinYeast cell wall contains polymers glucan and mannan-protein that have received much attention with respect to their biological activities. Conventional isolation process involving treatments with hot alkali and acids cause degradation of these polymers. The aim of this paper was to study a low-degrading process for the isolation of glucan and mannan-protein from S. cerevisiae cell wall comprising physic and enzymatic treatments. Yeast cell glucan was obtained in a purity of 87.4% and a yield of 33.7%. The isolated mannan-protein presented antioxidant activity that was increased after thirty minutes of protease treatment. Antioxidant activity was determined by β-carotene/linoleate model system.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200010Química Nova v.32 n.2 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000200010info:eu-repo/semantics/openAccessFerracini-Santos,LucianaSato,Hélia Harumipor2009-04-23T00:00:00Zoai:scielo:S0100-40422009000200010Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-04-23T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
title Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
spellingShingle Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
Ferracini-Santos,Luciana
yeast glucan
antioxidant activity
mannan-protein
title_short Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
title_full Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
title_fullStr Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
title_full_unstemmed Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
title_sort Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada
author Ferracini-Santos,Luciana
author_facet Ferracini-Santos,Luciana
Sato,Hélia Harumi
author_role author
author2 Sato,Hélia Harumi
author2_role author
dc.contributor.author.fl_str_mv Ferracini-Santos,Luciana
Sato,Hélia Harumi
dc.subject.por.fl_str_mv yeast glucan
antioxidant activity
mannan-protein
topic yeast glucan
antioxidant activity
mannan-protein
description Yeast cell wall contains polymers glucan and mannan-protein that have received much attention with respect to their biological activities. Conventional isolation process involving treatments with hot alkali and acids cause degradation of these polymers. The aim of this paper was to study a low-degrading process for the isolation of glucan and mannan-protein from S. cerevisiae cell wall comprising physic and enzymatic treatments. Yeast cell glucan was obtained in a purity of 87.4% and a yield of 33.7%. The isolated mannan-protein presented antioxidant activity that was increased after thirty minutes of protease treatment. Antioxidant activity was determined by β-carotene/linoleate model system.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000200010
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422009000200010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.32 n.2 2009
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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