High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma

Detalhes bibliográficos
Autor(a) principal: Conrado,Daniela J.
Data de Publicação: 2007
Outros Autores: Rates,Stela Maris Kuze, Costa,Teresa Dalla, Fraga,Carlos Alberto Manssour, Barreiro,Eliezer J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000800023
Resumo: A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio-579in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate - methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 mg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 mg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.
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spelling High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasmaLASSBio-579HPLCpharmacokineticsA rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio-579in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate - methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 mg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 mg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.Sociedade Brasileira de Química2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000800023Química Nova v.30 n.8 2007reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422007000800023info:eu-repo/semantics/openAccessConrado,Daniela J.Rates,Stela Maris KuzeCosta,Teresa DallaFraga,Carlos Alberto ManssourBarreiro,Eliezer J.eng2008-01-22T00:00:00Zoai:scielo:S0100-40422007000800023Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2008-01-22T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
title High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
spellingShingle High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
Conrado,Daniela J.
LASSBio-579
HPLC
pharmacokinetics
title_short High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
title_full High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
title_fullStr High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
title_full_unstemmed High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
title_sort High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
author Conrado,Daniela J.
author_facet Conrado,Daniela J.
Rates,Stela Maris Kuze
Costa,Teresa Dalla
Fraga,Carlos Alberto Manssour
Barreiro,Eliezer J.
author_role author
author2 Rates,Stela Maris Kuze
Costa,Teresa Dalla
Fraga,Carlos Alberto Manssour
Barreiro,Eliezer J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Conrado,Daniela J.
Rates,Stela Maris Kuze
Costa,Teresa Dalla
Fraga,Carlos Alberto Manssour
Barreiro,Eliezer J.
dc.subject.por.fl_str_mv LASSBio-579
HPLC
pharmacokinetics
topic LASSBio-579
HPLC
pharmacokinetics
description A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio-579in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate - methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 mg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 mg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000800023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000800023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422007000800023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.30 n.8 2007
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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