High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma

Detalhes bibliográficos
Autor(a) principal: Conrado, Daniela Joice
Data de Publicação: 2007
Outros Autores: Rates, Stela Maris Kuze, Dalla Costa, Teresa Cristina Tavares, Fraga, Carlos Alberto Manssour, Barreiro, Eliezer J.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/72862
Resumo: A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio- 579 in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate-methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 μg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 μg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.
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spelling Conrado, Daniela JoiceRates, Stela Maris KuzeDalla Costa, Teresa Cristina TavaresFraga, Carlos Alberto ManssourBarreiro, Eliezer J.2013-07-02T01:43:13Z20070100-4042http://hdl.handle.net/10183/72862000625742A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio- 579 in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate-methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 μg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 μg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.application/pdfporQuímica nova. São Paulo. Vol. 30, n. 8 (2007), p. 1019-1922Cromatografia liquida de alta eficiencia (clae)LASSBio-579FarmacocinéticaLASSBio-579HPLCPharmacokineticsHigh performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasmainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000625742.pdf000625742.pdfTexto completo (inglês)application/pdf89212http://www.lume.ufrgs.br/bitstream/10183/72862/1/000625742.pdfd04f83360e8f037a7c12411716e686b7MD51TEXT000625742.pdf.txt000625742.pdf.txtExtracted Texttext/plain18113http://www.lume.ufrgs.br/bitstream/10183/72862/2/000625742.pdf.txt0f1272372aedaff82d9623a6784bcadfMD52THUMBNAIL000625742.pdf.jpg000625742.pdf.jpgGenerated Thumbnailimage/jpeg1921http://www.lume.ufrgs.br/bitstream/10183/72862/3/000625742.pdf.jpgd5591dd4222e4b5a7b9b809076af6023MD5310183/728622018-10-15 08:10:36.232oai:www.lume.ufrgs.br:10183/72862Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-15T11:10:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
title High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
spellingShingle High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
Conrado, Daniela Joice
Cromatografia liquida de alta eficiencia (clae)
LASSBio-579
Farmacocinética
LASSBio-579
HPLC
Pharmacokinetics
title_short High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
title_full High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
title_fullStr High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
title_full_unstemmed High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
title_sort High performance liquid chromatography method for quantification of the n-phenylpiperazine derivative lassbio-579 in rat plasma
author Conrado, Daniela Joice
author_facet Conrado, Daniela Joice
Rates, Stela Maris Kuze
Dalla Costa, Teresa Cristina Tavares
Fraga, Carlos Alberto Manssour
Barreiro, Eliezer J.
author_role author
author2 Rates, Stela Maris Kuze
Dalla Costa, Teresa Cristina Tavares
Fraga, Carlos Alberto Manssour
Barreiro, Eliezer J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Conrado, Daniela Joice
Rates, Stela Maris Kuze
Dalla Costa, Teresa Cristina Tavares
Fraga, Carlos Alberto Manssour
Barreiro, Eliezer J.
dc.subject.por.fl_str_mv Cromatografia liquida de alta eficiencia (clae)
LASSBio-579
Farmacocinética
topic Cromatografia liquida de alta eficiencia (clae)
LASSBio-579
Farmacocinética
LASSBio-579
HPLC
Pharmacokinetics
dc.subject.eng.fl_str_mv LASSBio-579
HPLC
Pharmacokinetics
description A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio- 579 in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate-methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 μg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 μg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2013-07-02T01:43:13Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/72862
dc.identifier.issn.pt_BR.fl_str_mv 0100-4042
dc.identifier.nrb.pt_BR.fl_str_mv 000625742
identifier_str_mv 0100-4042
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url http://hdl.handle.net/10183/72862
dc.language.iso.fl_str_mv por
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dc.relation.ispartof.pt_BR.fl_str_mv Química nova. São Paulo. Vol. 30, n. 8 (2007), p. 1019-1922
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instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
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collection Repositório Institucional da UFRGS
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