Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies

Detalhes bibliográficos
Autor(a) principal: Fonseca,Yris Maria
Data de Publicação: 2010
Outros Autores: Vicentini,Fabiana T. M. C., Catini,Carolina Dias, Fonseca,Maria José Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019
Resumo: A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.
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spelling Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studiesCalendula officinalisnarcissinrutinA chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019Química Nova v.33 n.6 2010reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422010000600019info:eu-repo/semantics/openAccessFonseca,Yris MariaVicentini,Fabiana T. M. C.Catini,Carolina DiasFonseca,Maria José Vieiraeng2010-08-24T00:00:00Zoai:scielo:S0100-40422010000600019Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2010-08-24T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
title Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
spellingShingle Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
Fonseca,Yris Maria
Calendula officinalis
narcissin
rutin
title_short Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
title_full Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
title_fullStr Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
title_full_unstemmed Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
title_sort Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
author Fonseca,Yris Maria
author_facet Fonseca,Yris Maria
Vicentini,Fabiana T. M. C.
Catini,Carolina Dias
Fonseca,Maria José Vieira
author_role author
author2 Vicentini,Fabiana T. M. C.
Catini,Carolina Dias
Fonseca,Maria José Vieira
author2_role author
author
author
dc.contributor.author.fl_str_mv Fonseca,Yris Maria
Vicentini,Fabiana T. M. C.
Catini,Carolina Dias
Fonseca,Maria José Vieira
dc.subject.por.fl_str_mv Calendula officinalis
narcissin
rutin
topic Calendula officinalis
narcissin
rutin
description A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000600019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422010000600019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.33 n.6 2010
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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