A convenient method for lecithin purification from fresh eggs

Detalhes bibliográficos
Autor(a) principal: Maximiano,Flavio A.
Data de Publicação: 2008
Outros Autores: Silva,Marcia A. da, Daghastanli,Katia R. P., Araujo,Pedro S. de, Chaimovich,Hernan, Cuccovia,Iolanda M.
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000400032
Resumo: The increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with ethanol and solvent elimination and c) repeated solubilization/precipitation with petroleum ether/acetone. This crude extract was chromatographed on neutral alumina, which was exhaustively washed with chloroform before elution with chloroform:methanol, allowing the sequential separation of fatty acids and lecithin. Chromatographic behavior and mass spectra of the product are presented. This fast procedure yields fatty acid-free lecithin at a competitive cost.
id SBQ-3_a3e3eefe68d28e6f0b5f557c6629ab99
oai_identifier_str oai:scielo:S0100-40422008000400032
network_acronym_str SBQ-3
network_name_str Química Nova (Online)
repository_id_str
spelling A convenient method for lecithin purification from fresh eggslecithinpurificationphosphatidylcholineThe increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with ethanol and solvent elimination and c) repeated solubilization/precipitation with petroleum ether/acetone. This crude extract was chromatographed on neutral alumina, which was exhaustively washed with chloroform before elution with chloroform:methanol, allowing the sequential separation of fatty acids and lecithin. Chromatographic behavior and mass spectra of the product are presented. This fast procedure yields fatty acid-free lecithin at a competitive cost.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000400032Química Nova v.31 n.4 2008reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422008000400032info:eu-repo/semantics/openAccessMaximiano,Flavio A.Silva,Marcia A. daDaghastanli,Katia R. P.Araujo,Pedro S. deChaimovich,HernanCuccovia,Iolanda M.eng2008-06-16T00:00:00Zoai:scielo:S0100-40422008000400032Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2008-06-16T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A convenient method for lecithin purification from fresh eggs
title A convenient method for lecithin purification from fresh eggs
spellingShingle A convenient method for lecithin purification from fresh eggs
Maximiano,Flavio A.
lecithin
purification
phosphatidylcholine
title_short A convenient method for lecithin purification from fresh eggs
title_full A convenient method for lecithin purification from fresh eggs
title_fullStr A convenient method for lecithin purification from fresh eggs
title_full_unstemmed A convenient method for lecithin purification from fresh eggs
title_sort A convenient method for lecithin purification from fresh eggs
author Maximiano,Flavio A.
author_facet Maximiano,Flavio A.
Silva,Marcia A. da
Daghastanli,Katia R. P.
Araujo,Pedro S. de
Chaimovich,Hernan
Cuccovia,Iolanda M.
author_role author
author2 Silva,Marcia A. da
Daghastanli,Katia R. P.
Araujo,Pedro S. de
Chaimovich,Hernan
Cuccovia,Iolanda M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Maximiano,Flavio A.
Silva,Marcia A. da
Daghastanli,Katia R. P.
Araujo,Pedro S. de
Chaimovich,Hernan
Cuccovia,Iolanda M.
dc.subject.por.fl_str_mv lecithin
purification
phosphatidylcholine
topic lecithin
purification
phosphatidylcholine
description The increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with ethanol and solvent elimination and c) repeated solubilization/precipitation with petroleum ether/acetone. This crude extract was chromatographed on neutral alumina, which was exhaustively washed with chloroform before elution with chloroform:methanol, allowing the sequential separation of fatty acids and lecithin. Chromatographic behavior and mass spectra of the product are presented. This fast procedure yields fatty acid-free lecithin at a competitive cost.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000400032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000400032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422008000400032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.31 n.4 2008
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318107620016128

Registros relacionados