Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

• Autor(a) principal: Cid,Yara P.
• Data de Publicação: 2012
• Outros Autores: Ferreira,Thais P., Medeiros,Deborah M. V. C., Oliveira,Rodrigo M., Silva,Nathália C. C., Magalhães,Viviane S., Scott,Fábio B.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001000029
• topic: fipronil; bovine plasma; LC-UV

A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.