Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide

Detalhes bibliográficos
Autor(a) principal: Bruxel,Fernanda
Data de Publicação: 2011
Outros Autores: Guterres,Silvia S, Teixeira,Helder F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000900026
Resumo: This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions
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spelling Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotideantimalarial oligonucleotidesnanoemulsionsUV analysisThis study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsionsSociedade Brasileira de Química2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000900026Química Nova v.34 n.9 2011reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422011000900026info:eu-repo/semantics/openAccessBruxel,FernandaGuterres,Silvia STeixeira,Helder Feng2011-11-16T00:00:00Zoai:scielo:S0100-40422011000900026Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2011-11-16T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
spellingShingle Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
Bruxel,Fernanda
antimalarial oligonucleotides
nanoemulsions
UV analysis
title_short Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_full Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_fullStr Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_full_unstemmed Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_sort Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
author Bruxel,Fernanda
author_facet Bruxel,Fernanda
Guterres,Silvia S
Teixeira,Helder F
author_role author
author2 Guterres,Silvia S
Teixeira,Helder F
author2_role author
author
dc.contributor.author.fl_str_mv Bruxel,Fernanda
Guterres,Silvia S
Teixeira,Helder F
dc.subject.por.fl_str_mv antimalarial oligonucleotides
nanoemulsions
UV analysis
topic antimalarial oligonucleotides
nanoemulsions
UV analysis
description This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000900026
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000900026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422011000900026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.34 n.9 2011
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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