Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide

Detalhes bibliográficos
Autor(a) principal: Bruxel, Fernanda
Data de Publicação: 2011
Outros Autores: Guterres, Silvia Stanisçuaski, Teixeira, Helder Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/72914
Resumo: This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions.
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spelling Bruxel, FernandaGuterres, Silvia StanisçuaskiTeixeira, Helder Ferreira2013-07-02T01:43:36Z20110100-4042http://hdl.handle.net/10183/72914000820772This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions.application/pdfengQuímica nova. São Paulo. Vol. 34, n. 9 (2011), p. 1643-1646NanoemulsõesEspectrofotometriaOligonucleotídeosValidação : Métodos analíticosAntimalarial oligonucleotidesNanoemulsionsUV analysisValidation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotideinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000820772.pdf000820772.pdfTexto completo (inglês)application/pdf291716http://www.lume.ufrgs.br/bitstream/10183/72914/1/000820772.pdf8d53308381510f5c32115a4826250feeMD51TEXT000820772.pdf.txt000820772.pdf.txtExtracted Texttext/plain20375http://www.lume.ufrgs.br/bitstream/10183/72914/2/000820772.pdf.txt32b7ec4c61e1a3626d33612d1a06083aMD52THUMBNAIL000820772.pdf.jpg000820772.pdf.jpgGenerated Thumbnailimage/jpeg1830http://www.lume.ufrgs.br/bitstream/10183/72914/3/000820772.pdf.jpg5643bc8be964ce89d839f7584171a64bMD5310183/729142018-10-15 08:04:57.483oai:www.lume.ufrgs.br:10183/72914Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-15T11:04:57Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
spellingShingle Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
Bruxel, Fernanda
Nanoemulsões
Espectrofotometria
Oligonucleotídeos
Validação : Métodos analíticos
Antimalarial oligonucleotides
Nanoemulsions
UV analysis
title_short Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_full Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_fullStr Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_full_unstemmed Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
title_sort Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide
author Bruxel, Fernanda
author_facet Bruxel, Fernanda
Guterres, Silvia Stanisçuaski
Teixeira, Helder Ferreira
author_role author
author2 Guterres, Silvia Stanisçuaski
Teixeira, Helder Ferreira
author2_role author
author
dc.contributor.author.fl_str_mv Bruxel, Fernanda
Guterres, Silvia Stanisçuaski
Teixeira, Helder Ferreira
dc.subject.por.fl_str_mv Nanoemulsões
Espectrofotometria
Oligonucleotídeos
Validação : Métodos analíticos
topic Nanoemulsões
Espectrofotometria
Oligonucleotídeos
Validação : Métodos analíticos
Antimalarial oligonucleotides
Nanoemulsions
UV analysis
dc.subject.eng.fl_str_mv Antimalarial oligonucleotides
Nanoemulsions
UV analysis
description This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2013-07-02T01:43:36Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/72914
dc.identifier.issn.pt_BR.fl_str_mv 0100-4042
dc.identifier.nrb.pt_BR.fl_str_mv 000820772
identifier_str_mv 0100-4042
000820772
url http://hdl.handle.net/10183/72914
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Química nova. São Paulo. Vol. 34, n. 9 (2011), p. 1643-1646
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