Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico

Detalhes bibliográficos
Autor(a) principal: Soares,Cleide Mara Faria
Data de Publicação: 2003
Outros Autores: Santana,Maria Helena Andrade, Zanin,Gisella M., Castro,Heizir F. de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422003000600010
Resumo: Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.
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spelling Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânicoadditivescontrolled pore silicaimmobilized lipasePoly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.Sociedade Brasileira de Química2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422003000600010Química Nova v.26 n.6 2003reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422003000600010info:eu-repo/semantics/openAccessSoares,Cleide Mara FariaSantana,Maria Helena AndradeZanin,Gisella M.Castro,Heizir F. depor2003-12-03T00:00:00Zoai:scielo:S0100-40422003000600010Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2003-12-03T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
title Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
spellingShingle Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
Soares,Cleide Mara Faria
additives
controlled pore silica
immobilized lipase
title_short Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
title_full Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
title_fullStr Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
title_full_unstemmed Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
title_sort Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
author Soares,Cleide Mara Faria
author_facet Soares,Cleide Mara Faria
Santana,Maria Helena Andrade
Zanin,Gisella M.
Castro,Heizir F. de
author_role author
author2 Santana,Maria Helena Andrade
Zanin,Gisella M.
Castro,Heizir F. de
author2_role author
author
author
dc.contributor.author.fl_str_mv Soares,Cleide Mara Faria
Santana,Maria Helena Andrade
Zanin,Gisella M.
Castro,Heizir F. de
dc.subject.por.fl_str_mv additives
controlled pore silica
immobilized lipase
topic additives
controlled pore silica
immobilized lipase
description Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422003000600010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422003000600010
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422003000600010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.26 n.6 2003
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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