Otimização de método para análise de folatos em hortaliças folhosas por cromatografia líquida de alta eficiência com detecção por fluorescência

• Main Author: Lucia,Ceres Mattos Della
• Publication Date: 2011
• Other Authors: Silva,Elizangela Rodrigues da, Ribeiro,Sônia Machado Rocha, Pinheiro-Sant'Ana,Helena Maria, Brandão,Sebastião César Cardoso
• Format: Article
• Language: por
• Source: Química Nova (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000200029
• topic: tetrahydrofolate; 5-methyltetrahydrofolate; 5-formyltetrahydrofolate

We optimized a method for the determination of three main folate isoforms in leafy vegetables. Analysis was carried out by HPLC, using gradient of elution and fluorescence detection. Limits of quantification were 2 ng/mL; 2 ng/mL and 28 ng/mL for tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF), respectively. Recovery rates of THF, 5-MTHF and 5-FTHF varied from 87.86 to 100.64%; 88.12-94.22% and 89.82-106.69%, respectively. Test of repeatability showed relative standard deviation for peak areas lower than 10%. Tests for linearity showed high coefficients of determination. The results showed advances in folates extraction and analysis in raw and cooked leafy vegetables.