ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA

Detalhes bibliográficos
Autor(a) principal: Cruz,Alessandro de C.
Data de Publicação: 2017
Outros Autores: Suenaga,Eunice M., Abib,Eduardo, Pedrazzoli,José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000100025
Resumo: A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using on-line solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing high-throughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL-1. Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.
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spelling ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMAon-line SPELC-MS/MShigh-throughputmonolithic columncodeineA specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using on-line solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing high-throughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL-1. Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.Sociedade Brasileira de Química2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000100025Química Nova v.40 n.1 2017reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20160153info:eu-repo/semantics/openAccessCruz,Alessandro de C.Suenaga,Eunice M.Abib,EduardoPedrazzoli,Joséeng2017-02-24T00:00:00Zoai:scielo:S0100-40422017000100025Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2017-02-24T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
title ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
spellingShingle ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
Cruz,Alessandro de C.
on-line SPE
LC-MS/MS
high-throughput
monolithic column
codeine
title_short ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
title_full ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
title_fullStr ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
title_full_unstemmed ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
title_sort ON-LINE SOLID PHASE EXTRACTION COUPLED WITH LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY FOR THE DETERMINATION OF CODEINE IN HUMAN PLASMA
author Cruz,Alessandro de C.
author_facet Cruz,Alessandro de C.
Suenaga,Eunice M.
Abib,Eduardo
Pedrazzoli,José
author_role author
author2 Suenaga,Eunice M.
Abib,Eduardo
Pedrazzoli,José
author2_role author
author
author
dc.contributor.author.fl_str_mv Cruz,Alessandro de C.
Suenaga,Eunice M.
Abib,Eduardo
Pedrazzoli,José
dc.subject.por.fl_str_mv on-line SPE
LC-MS/MS
high-throughput
monolithic column
codeine
topic on-line SPE
LC-MS/MS
high-throughput
monolithic column
codeine
description A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using on-line solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing high-throughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL-1. Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000100025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000100025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0100-4042.20160153
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.40 n.1 2017
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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