Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules

Detalhes bibliográficos
Autor(a) principal: Silva,Breno de Carvalho e
Data de Publicação: 2005
Outros Autores: Campos,Lígia Maria Moreira de, Nunan,Elzíria Aguiar, Soares,Cristina Duarte Vianna, Silva,Gisele Rodrigues, Ribeiro,Jóse Antônio de Aquino, Pianetti,Gerson Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000100010
Resumo: Two high performance liquid chromatography (HPLC) methods for the quantitative determination of indinavir sulfate were tested, validated and statistically compared. Assays were carried out using as mobile phases mixtures of dibutylammonium phosphate buffer pH 6.5 and acetonitrile (55:45) at 1 mL/min or citrate buffer pH 5 and acetonitrile (60:40) at 1 mL/min, an octylsilane column (RP-8) and a UV spectrophotometric detector at 260 nm. Both methods showed good sensitivity, linearity, precision and accuracy. The statistical analysis using the t-student test for the determination of indinavir sulfate raw material and capsules indicated no statistically significant difference between the two methods.
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spelling Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsulesindinavir sulfateHPLCvalidationTwo high performance liquid chromatography (HPLC) methods for the quantitative determination of indinavir sulfate were tested, validated and statistically compared. Assays were carried out using as mobile phases mixtures of dibutylammonium phosphate buffer pH 6.5 and acetonitrile (55:45) at 1 mL/min or citrate buffer pH 5 and acetonitrile (60:40) at 1 mL/min, an octylsilane column (RP-8) and a UV spectrophotometric detector at 260 nm. Both methods showed good sensitivity, linearity, precision and accuracy. The statistical analysis using the t-student test for the determination of indinavir sulfate raw material and capsules indicated no statistically significant difference between the two methods.Sociedade Brasileira de Química2005-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000100010Química Nova v.28 n.1 2005reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422005000100010info:eu-repo/semantics/openAccessSilva,Breno de Carvalho eCampos,Lígia Maria Moreira deNunan,Elzíria AguiarSoares,Cristina Duarte ViannaSilva,Gisele RodriguesRibeiro,Jóse Antônio de AquinoPianetti,Gerson Antônioeng2005-02-23T00:00:00Zoai:scielo:S0100-40422005000100010Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2005-02-23T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
title Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
spellingShingle Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
Silva,Breno de Carvalho e
indinavir sulfate
HPLC
validation
title_short Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
title_full Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
title_fullStr Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
title_full_unstemmed Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
title_sort Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules
author Silva,Breno de Carvalho e
author_facet Silva,Breno de Carvalho e
Campos,Lígia Maria Moreira de
Nunan,Elzíria Aguiar
Soares,Cristina Duarte Vianna
Silva,Gisele Rodrigues
Ribeiro,Jóse Antônio de Aquino
Pianetti,Gerson Antônio
author_role author
author2 Campos,Lígia Maria Moreira de
Nunan,Elzíria Aguiar
Soares,Cristina Duarte Vianna
Silva,Gisele Rodrigues
Ribeiro,Jóse Antônio de Aquino
Pianetti,Gerson Antônio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Breno de Carvalho e
Campos,Lígia Maria Moreira de
Nunan,Elzíria Aguiar
Soares,Cristina Duarte Vianna
Silva,Gisele Rodrigues
Ribeiro,Jóse Antônio de Aquino
Pianetti,Gerson Antônio
dc.subject.por.fl_str_mv indinavir sulfate
HPLC
validation
topic indinavir sulfate
HPLC
validation
description Two high performance liquid chromatography (HPLC) methods for the quantitative determination of indinavir sulfate were tested, validated and statistically compared. Assays were carried out using as mobile phases mixtures of dibutylammonium phosphate buffer pH 6.5 and acetonitrile (55:45) at 1 mL/min or citrate buffer pH 5 and acetonitrile (60:40) at 1 mL/min, an octylsilane column (RP-8) and a UV spectrophotometric detector at 260 nm. Both methods showed good sensitivity, linearity, precision and accuracy. The statistical analysis using the t-student test for the determination of indinavir sulfate raw material and capsules indicated no statistically significant difference between the two methods.
publishDate 2005
dc.date.none.fl_str_mv 2005-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000100010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422005000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.28 n.1 2005
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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