Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000800010 |
Resumo: | The marine diatom Coscinodiscus wailesii has attracted ecological interest because their blooms affect fishing areas. The aim of this work was the isolation, extraction and partial chemical characterization of soluble exopolysaccharide and bound exopolysaccharide from C. wailesii. Cultures were grown in Guillards f/2 medium under controlled conditions of temperature, aeration, photoperiod and light intensity. Percentage of carbohydrate, uronic acids, sulfates groups and cellular lipids was determined. Ion exchange chromatography of exopolysaccharides produced three fractions whose partial chemical structures were disclosed using 13C NMR and methylation techniques. |
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Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta)Coscinodiscus wailesiidiatomexopolysaccharidesThe marine diatom Coscinodiscus wailesii has attracted ecological interest because their blooms affect fishing areas. The aim of this work was the isolation, extraction and partial chemical characterization of soluble exopolysaccharide and bound exopolysaccharide from C. wailesii. Cultures were grown in Guillards f/2 medium under controlled conditions of temperature, aeration, photoperiod and light intensity. Percentage of carbohydrate, uronic acids, sulfates groups and cellular lipids was determined. Ion exchange chromatography of exopolysaccharides produced three fractions whose partial chemical structures were disclosed using 13C NMR and methylation techniques.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000800010Química Nova v.35 n.8 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012000800010info:eu-repo/semantics/openAccessMarson-Ascêncio,Poliana G.Ascêncio,Sérgio DonizetiBaggio,Selma Faria Zawadzkipor2012-09-04T00:00:00Zoai:scielo:S0100-40422012000800010Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-09-04T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
title |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
spellingShingle |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) Marson-Ascêncio,Poliana G. Coscinodiscus wailesii diatom exopolysaccharides |
title_short |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
title_full |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
title_fullStr |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
title_full_unstemmed |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
title_sort |
Isolamento e análise química parcial de exopolissacarídeos da diatomácea marinha cultivada Coscinodiscus wailesii (Coscinodiscales, Bacillariophyta) |
author |
Marson-Ascêncio,Poliana G. |
author_facet |
Marson-Ascêncio,Poliana G. Ascêncio,Sérgio Donizeti Baggio,Selma Faria Zawadzki |
author_role |
author |
author2 |
Ascêncio,Sérgio Donizeti Baggio,Selma Faria Zawadzki |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Marson-Ascêncio,Poliana G. Ascêncio,Sérgio Donizeti Baggio,Selma Faria Zawadzki |
dc.subject.por.fl_str_mv |
Coscinodiscus wailesii diatom exopolysaccharides |
topic |
Coscinodiscus wailesii diatom exopolysaccharides |
description |
The marine diatom Coscinodiscus wailesii has attracted ecological interest because their blooms affect fishing areas. The aim of this work was the isolation, extraction and partial chemical characterization of soluble exopolysaccharide and bound exopolysaccharide from C. wailesii. Cultures were grown in Guillards f/2 medium under controlled conditions of temperature, aeration, photoperiod and light intensity. Percentage of carbohydrate, uronic acids, sulfates groups and cellular lipids was determined. Ion exchange chromatography of exopolysaccharides produced three fractions whose partial chemical structures were disclosed using 13C NMR and methylation techniques. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000800010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000800010 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.1590/S0100-40422012000800010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.35 n.8 2012 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318113891549184 |