Composição química e atividade anticolinesterásica de uma fração ativa do extrato de folhas de Citrus limon (L.) Burm

• Autor(a) principal: Carvalho,Rusbene Bruno Fonseca de
• Data de Publicação: 2013
• Outros Autores: Almeida,Antonia Amanda Cardoso de, Freitas,Rivelilson Mendes de, Lima,Luciano Silva, David,Juceni Pereira, David,Jorge Mauricio, Feitosa,Chistiane Mendes
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000900017
• topic: Citrus limon (L.) Burm; psoraleno; acetylcholinesterase

This paper describes the chemical composition and acetylcholinesterase inhibition of an active chromatographic fraction of the EtOAc extract of Citrus limon leaves. The composition of the active fraction presented a mixture of two coumarins, 5,8-dimethoxy-psoralen and 5,7- dimethoxycoumarin, identified by ¹H and ¹³C NMR data analysis, including DEPT, COSY, HMBC and HSQC experiments. It was also demonstrated that this mixture presents qualitative and quantitative AChE inhibition. In vitro studies indicated a CE50 value of 340 µg/mL with 95% of confidence. In vivo studies (10 and 25 mg/kg) revealed inhibition of 30.09 and 30.06% of AChE activity in relation to neostigmine, respectively.