Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Botany |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000400006 |
Resumo: | Increasing levels of atmospheric ammonia from anthropogenic sources have become a serious problem for natural vegetation. Short-term effects of different ammoniacal sources on the N metabolism of Tillandsia pohliana, an atmospheric bromeliad, were investigated. One-year-old, aseptically grown plants were transferred to a modified Knudson medium lacking N for three weeks. Plants were subsequently transferred to Knudson media supplemented with 0.5, 1.0, or 1.5 mM of N in the forms of NH3 or NH4+ as the sole N source. The activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH-NADH) were determined after 40 h. The GS activity was stimulated significantly by increasing the levels of the gaseous form. The GDH-NADH activity increased significantly under increasing N concentrations with NH3, while no significant differences were observed with NH4+ as a N source. These results may reflect a faster NH3 absorption by T. pohliana compared to NH4+ uptake. The increased activity of GDH-NADH in NH3 treatment may play a role in protecting the cells from the toxic effects of increased endogenous level of free ammonium. A raise in the concentration of N, especially in the form of NH3, greatly increased the content of free amino acids and soluble proteins. A possible utilisation of T. pohliana to evaluate the changes of atmospheric gaseous ammonia is proposed. |
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Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana MezBromeliaceaeNH3, NH4+GSGDH-NADHIncreasing levels of atmospheric ammonia from anthropogenic sources have become a serious problem for natural vegetation. Short-term effects of different ammoniacal sources on the N metabolism of Tillandsia pohliana, an atmospheric bromeliad, were investigated. One-year-old, aseptically grown plants were transferred to a modified Knudson medium lacking N for three weeks. Plants were subsequently transferred to Knudson media supplemented with 0.5, 1.0, or 1.5 mM of N in the forms of NH3 or NH4+ as the sole N source. The activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH-NADH) were determined after 40 h. The GS activity was stimulated significantly by increasing the levels of the gaseous form. The GDH-NADH activity increased significantly under increasing N concentrations with NH3, while no significant differences were observed with NH4+ as a N source. These results may reflect a faster NH3 absorption by T. pohliana compared to NH4+ uptake. The increased activity of GDH-NADH in NH3 treatment may play a role in protecting the cells from the toxic effects of increased endogenous level of free ammonium. A raise in the concentration of N, especially in the form of NH3, greatly increased the content of free amino acids and soluble proteins. A possible utilisation of T. pohliana to evaluate the changes of atmospheric gaseous ammonia is proposed.Sociedade Botânica de São Paulo2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000400006Brazilian Journal of Botany v.24 n.4 2001reponame:Brazilian Journal of Botanyinstname:Sociedade Botânica de São Paulo (SBSP)instacron:SBSP10.1590/S0100-84042001000400006info:eu-repo/semantics/openAccessTAMAKI,VÍVIANMERCIER,HELENICEeng2002-07-31T00:00:00Zoai:scielo:S0100-84042001000400006Revistahttps://www.scielo.br/j/rbb/ONGhttps://old.scielo.br/oai/scielo-oai.phpbrazbot@gmail.com||brazbot@gmail.com1806-99590100-8404opendoar:2002-07-31T00:00Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP)false |
dc.title.none.fl_str_mv |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
title |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
spellingShingle |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez TAMAKI,VÍVIAN Bromeliaceae NH3, NH4+ GS GDH-NADH |
title_short |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
title_full |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
title_fullStr |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
title_full_unstemmed |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
title_sort |
Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez |
author |
TAMAKI,VÍVIAN |
author_facet |
TAMAKI,VÍVIAN MERCIER,HELENICE |
author_role |
author |
author2 |
MERCIER,HELENICE |
author2_role |
author |
dc.contributor.author.fl_str_mv |
TAMAKI,VÍVIAN MERCIER,HELENICE |
dc.subject.por.fl_str_mv |
Bromeliaceae NH3, NH4+ GS GDH-NADH |
topic |
Bromeliaceae NH3, NH4+ GS GDH-NADH |
description |
Increasing levels of atmospheric ammonia from anthropogenic sources have become a serious problem for natural vegetation. Short-term effects of different ammoniacal sources on the N metabolism of Tillandsia pohliana, an atmospheric bromeliad, were investigated. One-year-old, aseptically grown plants were transferred to a modified Knudson medium lacking N for three weeks. Plants were subsequently transferred to Knudson media supplemented with 0.5, 1.0, or 1.5 mM of N in the forms of NH3 or NH4+ as the sole N source. The activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH-NADH) were determined after 40 h. The GS activity was stimulated significantly by increasing the levels of the gaseous form. The GDH-NADH activity increased significantly under increasing N concentrations with NH3, while no significant differences were observed with NH4+ as a N source. These results may reflect a faster NH3 absorption by T. pohliana compared to NH4+ uptake. The increased activity of GDH-NADH in NH3 treatment may play a role in protecting the cells from the toxic effects of increased endogenous level of free ammonium. A raise in the concentration of N, especially in the form of NH3, greatly increased the content of free amino acids and soluble proteins. A possible utilisation of T. pohliana to evaluate the changes of atmospheric gaseous ammonia is proposed. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000400006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000400006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-84042001000400006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
dc.source.none.fl_str_mv |
Brazilian Journal of Botany v.24 n.4 2001 reponame:Brazilian Journal of Botany instname:Sociedade Botânica de São Paulo (SBSP) instacron:SBSP |
instname_str |
Sociedade Botânica de São Paulo (SBSP) |
instacron_str |
SBSP |
institution |
SBSP |
reponame_str |
Brazilian Journal of Botany |
collection |
Brazilian Journal of Botany |
repository.name.fl_str_mv |
Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP) |
repository.mail.fl_str_mv |
brazbot@gmail.com||brazbot@gmail.com |
_version_ |
1754734837808234496 |