Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004 |
Resumo: | The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore cows, mated to Canchim, Caracu and Red Angus bulls (only Caracu × Nellore cows were used with Red Angus bulls). The allelic and genotypic frequencies were compared using the Chi-squared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model (GLM) of statistical software SAS. The least squares means of genotypes of the polymorphisms were compared using Student's t test. The E2FB polymorphism in the LEP gene was associated with CFT, showing the potential for use in national programs for genetic improvement of beef cattle, through the inclusion of SNP in genotyping commercial tests. The TG5 polymorphism in the TG gene was not associated with any of the evaluated traits and was considered ineffective for selection of beef cattle in Brazilian herds. |
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Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattlefat thicknessmarker-assisted selectionmeat tendernessshear forceSNPThe objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore cows, mated to Canchim, Caracu and Red Angus bulls (only Caracu × Nellore cows were used with Red Angus bulls). The allelic and genotypic frequencies were compared using the Chi-squared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model (GLM) of statistical software SAS. The least squares means of genotypes of the polymorphisms were compared using Student's t test. The E2FB polymorphism in the LEP gene was associated with CFT, showing the potential for use in national programs for genetic improvement of beef cattle, through the inclusion of SNP in genotyping commercial tests. The TG5 polymorphism in the TG gene was not associated with any of the evaluated traits and was considered ineffective for selection of beef cattle in Brazilian herds.Sociedade Brasileira de Zootecnia2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004Revista Brasileira de Zootecnia v.41 n.10 2012reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982012001000004info:eu-repo/semantics/openAccessCarvalho,Thiago Dutra deSiqueira,FabianeTorres Júnior,Roberto Augusto de AlmeidaMedeiros,Sérgio Raposo deFeijó,Gelson Luís DiasSouza Junior,Maury Dorta deBlecha,Isabella Maiumi ZaidanSoares,Cleber Oliveiraeng2012-11-20T00:00:00Zoai:scielo:S1516-35982012001000004Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2012-11-20T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
title |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
spellingShingle |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle Carvalho,Thiago Dutra de fat thickness marker-assisted selection meat tenderness shear force SNP |
title_short |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
title_full |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
title_fullStr |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
title_full_unstemmed |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
title_sort |
Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle |
author |
Carvalho,Thiago Dutra de |
author_facet |
Carvalho,Thiago Dutra de Siqueira,Fabiane Torres Júnior,Roberto Augusto de Almeida Medeiros,Sérgio Raposo de Feijó,Gelson Luís Dias Souza Junior,Maury Dorta de Blecha,Isabella Maiumi Zaidan Soares,Cleber Oliveira |
author_role |
author |
author2 |
Siqueira,Fabiane Torres Júnior,Roberto Augusto de Almeida Medeiros,Sérgio Raposo de Feijó,Gelson Luís Dias Souza Junior,Maury Dorta de Blecha,Isabella Maiumi Zaidan Soares,Cleber Oliveira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho,Thiago Dutra de Siqueira,Fabiane Torres Júnior,Roberto Augusto de Almeida Medeiros,Sérgio Raposo de Feijó,Gelson Luís Dias Souza Junior,Maury Dorta de Blecha,Isabella Maiumi Zaidan Soares,Cleber Oliveira |
dc.subject.por.fl_str_mv |
fat thickness marker-assisted selection meat tenderness shear force SNP |
topic |
fat thickness marker-assisted selection meat tenderness shear force SNP |
description |
The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore cows, mated to Canchim, Caracu and Red Angus bulls (only Caracu × Nellore cows were used with Red Angus bulls). The allelic and genotypic frequencies were compared using the Chi-squared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model (GLM) of statistical software SAS. The least squares means of genotypes of the polymorphisms were compared using Student's t test. The E2FB polymorphism in the LEP gene was associated with CFT, showing the potential for use in national programs for genetic improvement of beef cattle, through the inclusion of SNP in genotyping commercial tests. The TG5 polymorphism in the TG gene was not associated with any of the evaluated traits and was considered ineffective for selection of beef cattle in Brazilian herds. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-35982012001000004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.41 n.10 2012 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
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1750318150394576896 |