Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)

Detalhes bibliográficos
Autor(a) principal: Huo,Shengdong
Data de Publicação: 2015
Outros Autores: Yuan,Qijian, Zhang,Taojie, Liu,Junlin, Abudureyimu,Ayimuguli, Yang,Jutian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350
Resumo: ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak.
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spelling Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)immunofluorescencereal-time PCRABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak.Sociedade Brasileira de Zootecnia2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350Revista Brasileira de Zootecnia v.44 n.10 2015reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1806-92902015001000002info:eu-repo/semantics/openAccessHuo,ShengdongYuan,QijianZhang,TaojieLiu,JunlinAbudureyimu,AyimuguliYang,Jutianeng2015-11-05T00:00:00Zoai:scielo:S1516-35982015001000350Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2015-11-05T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
title Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
spellingShingle Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
Huo,Shengdong
immunofluorescence
real-time PCR
title_short Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
title_full Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
title_fullStr Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
title_full_unstemmed Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
title_sort Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
author Huo,Shengdong
author_facet Huo,Shengdong
Yuan,Qijian
Zhang,Taojie
Liu,Junlin
Abudureyimu,Ayimuguli
Yang,Jutian
author_role author
author2 Yuan,Qijian
Zhang,Taojie
Liu,Junlin
Abudureyimu,Ayimuguli
Yang,Jutian
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Huo,Shengdong
Yuan,Qijian
Zhang,Taojie
Liu,Junlin
Abudureyimu,Ayimuguli
Yang,Jutian
dc.subject.por.fl_str_mv immunofluorescence
real-time PCR
topic immunofluorescence
real-time PCR
description ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-92902015001000002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.44 n.10 2015
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zootecnia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
repository.mail.fl_str_mv ||bz@sbz.org.br|| secretariarbz@sbz.org.br
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