Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
Resumo: | ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak. |
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Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)immunofluorescencereal-time PCRABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak.Sociedade Brasileira de Zootecnia2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350Revista Brasileira de Zootecnia v.44 n.10 2015reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1806-92902015001000002info:eu-repo/semantics/openAccessHuo,ShengdongYuan,QijianZhang,TaojieLiu,JunlinAbudureyimu,AyimuguliYang,Jutianeng2015-11-05T00:00:00Zoai:scielo:S1516-35982015001000350Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2015-11-05T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
title |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
spellingShingle |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) Huo,Shengdong immunofluorescence real-time PCR |
title_short |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
title_full |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
title_fullStr |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
title_full_unstemmed |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
title_sort |
Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens) |
author |
Huo,Shengdong |
author_facet |
Huo,Shengdong Yuan,Qijian Zhang,Taojie Liu,Junlin Abudureyimu,Ayimuguli Yang,Jutian |
author_role |
author |
author2 |
Yuan,Qijian Zhang,Taojie Liu,Junlin Abudureyimu,Ayimuguli Yang,Jutian |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Huo,Shengdong Yuan,Qijian Zhang,Taojie Liu,Junlin Abudureyimu,Ayimuguli Yang,Jutian |
dc.subject.por.fl_str_mv |
immunofluorescence real-time PCR |
topic |
immunofluorescence real-time PCR |
description |
ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the ovarian follicle, corpus luteum and Leydig cells. Estradiol exerts its main effects on the pituitary gland during estrus in yak. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-92902015001000002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.44 n.10 2015 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
_version_ |
1750318151730462720 |