Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus

Detalhes bibliográficos
Autor(a) principal: Huo,Sheng-dong
Data de Publicação: 2018
Outros Autores: Zhang,Tao-jie, Abudureyimu,Ayimuguli, Liu,Jun-lin, Zhang,Guo-hua, Ma,Zhong-ren
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401
Resumo: ABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle and Leydig cells. Gonadotropin-releasing hormone receptor is located in the pineal gland, hypothalamus, pituitary, and gonad during estrus of yaks and is mainly expressed in the hypothalamus and ovaries during the estrus period.
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spelling Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrusBos grunniensgonadotropin-releasing hormone receptorimmunofluorescencereal-time PCRABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle and Leydig cells. Gonadotropin-releasing hormone receptor is located in the pineal gland, hypothalamus, pituitary, and gonad during estrus of yaks and is mainly expressed in the hypothalamus and ovaries during the estrus period.Sociedade Brasileira de Zootecnia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401Revista Brasileira de Zootecnia v.47 2018reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/rbz4720160360info:eu-repo/semantics/openAccessHuo,Sheng-dongZhang,Tao-jieAbudureyimu,AyimuguliLiu,Jun-linZhang,Guo-huaMa,Zhong-reneng2018-02-08T00:00:00Zoai:scielo:S1516-35982018000100401Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2018-02-08T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
title Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
spellingShingle Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
Huo,Sheng-dong
Bos grunniens
gonadotropin-releasing hormone receptor
immunofluorescence
real-time PCR
title_short Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
title_full Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
title_fullStr Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
title_full_unstemmed Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
title_sort Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
author Huo,Sheng-dong
author_facet Huo,Sheng-dong
Zhang,Tao-jie
Abudureyimu,Ayimuguli
Liu,Jun-lin
Zhang,Guo-hua
Ma,Zhong-ren
author_role author
author2 Zhang,Tao-jie
Abudureyimu,Ayimuguli
Liu,Jun-lin
Zhang,Guo-hua
Ma,Zhong-ren
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Huo,Sheng-dong
Zhang,Tao-jie
Abudureyimu,Ayimuguli
Liu,Jun-lin
Zhang,Guo-hua
Ma,Zhong-ren
dc.subject.por.fl_str_mv Bos grunniens
gonadotropin-releasing hormone receptor
immunofluorescence
real-time PCR
topic Bos grunniens
gonadotropin-releasing hormone receptor
immunofluorescence
real-time PCR
description ABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle and Leydig cells. Gonadotropin-releasing hormone receptor is located in the pineal gland, hypothalamus, pituitary, and gonad during estrus of yaks and is mainly expressed in the hypothalamus and ovaries during the estrus period.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/rbz4720160360
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.47 2018
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zootecnia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
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