Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

Detalhes bibliográficos
Autor(a) principal: Lew,Betina Joyce
Data de Publicação: 2013
Outros Autores: Oliveira,Mauro Dal Secco de, Freitas Júnior,José Esler de, Carvalho,Marina Vieira de, Rêgo,Aníbal Coutinho do, Rennó,Francisco Palma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008
Resumo: The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet.
id SBZ-1_a0837881254856750a7e4bb56518939c
oai_identifier_str oai:scielo:S1516-35982013000700008
network_acronym_str SBZ-1
network_name_str Revista Brasileira de Zootecnia (Online)
repository_id_str
spelling Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifersgene regulationmammary gland developmentmicroarraynutritionsomatotropinThe objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet.Sociedade Brasileira de Zootecnia2013-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008Revista Brasileira de Zootecnia v.42 n.7 2013reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982013000700008info:eu-repo/semantics/openAccessLew,Betina JoyceOliveira,Mauro Dal Secco deFreitas Júnior,José Esler deCarvalho,Marina Vieira deRêgo,Aníbal Coutinho doRennó,Francisco Palmaeng2013-06-24T00:00:00Zoai:scielo:S1516-35982013000700008Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2013-06-24T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
title Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
spellingShingle Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
Lew,Betina Joyce
gene regulation
mammary gland development
microarray
nutrition
somatotropin
title_short Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
title_full Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
title_fullStr Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
title_full_unstemmed Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
title_sort Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
author Lew,Betina Joyce
author_facet Lew,Betina Joyce
Oliveira,Mauro Dal Secco de
Freitas Júnior,José Esler de
Carvalho,Marina Vieira de
Rêgo,Aníbal Coutinho do
Rennó,Francisco Palma
author_role author
author2 Oliveira,Mauro Dal Secco de
Freitas Júnior,José Esler de
Carvalho,Marina Vieira de
Rêgo,Aníbal Coutinho do
Rennó,Francisco Palma
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lew,Betina Joyce
Oliveira,Mauro Dal Secco de
Freitas Júnior,José Esler de
Carvalho,Marina Vieira de
Rêgo,Aníbal Coutinho do
Rennó,Francisco Palma
dc.subject.por.fl_str_mv gene regulation
mammary gland development
microarray
nutrition
somatotropin
topic gene regulation
mammary gland development
microarray
nutrition
somatotropin
description The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-35982013000700008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.42 n.7 2013
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zootecnia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
repository.mail.fl_str_mv ||bz@sbz.org.br|| secretariarbz@sbz.org.br
_version_ 1750318150861193216

Registros relacionados