Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

• Autor(a) principal: Lew, Betina Joyce
• Data de Publicação: 2013
• Outros Autores: de Oliveira, Mauro Dal Secco [UNESP], Júnior, José Esler de Freitas, de Carvalho, Marina Vieira, do Rêgo, Aníbal Coutinho [UNESP], Rennó, Francisco Palma
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1516-35982013000700008; http://hdl.handle.net/11449/75854
• topic: Gene regulation; Mammary gland development; Microarray; Nutrition; Somatotropin

The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA micro array (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet. © 2013 Sociedade Brasileira de Zootecnia.