Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Zoologia (Curitiba. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100335 |
Resumo: | ABSTRACT Planarians, the representatives of an ancient bilaterian group with complex reproductive system and high regenerative capabilities, are model system suitable for studying the basic molecular requirements for the development of the reproductive system. To further explore the morphological changes of the gonads during desexualization and the molecular events of the genes controlling the reproductive system development in planarians, we have investigated the histological changes of ovary and testis by paraffin section and the expression patterns of reproductive-related genes by the quantitative real-time PCR in Dugesia japonica Ichikawa & Kawakatsu, 1964, upon starvation. The four genes, Djprps, DjvlgA, DjvlgB and Djnos, have been selected. The research results show that the degradation of ovary changes from outside layer to inside, and the testis changes are opposite; the reproductive capacity of the planarians starts to be damaged from the 17th to 25th days and to disappear completely from the 26th to 37th days during starvation. The expression patterns of the four genes exhibit the obvious dynamic variations during their desexualization, which indicates that these genes might be involved in gonad development. |
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Zoologia (Curitiba. Online) |
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Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae)Planarianfood deprivationgerm cellsreproductive systemABSTRACT Planarians, the representatives of an ancient bilaterian group with complex reproductive system and high regenerative capabilities, are model system suitable for studying the basic molecular requirements for the development of the reproductive system. To further explore the morphological changes of the gonads during desexualization and the molecular events of the genes controlling the reproductive system development in planarians, we have investigated the histological changes of ovary and testis by paraffin section and the expression patterns of reproductive-related genes by the quantitative real-time PCR in Dugesia japonica Ichikawa & Kawakatsu, 1964, upon starvation. The four genes, Djprps, DjvlgA, DjvlgB and Djnos, have been selected. The research results show that the degradation of ovary changes from outside layer to inside, and the testis changes are opposite; the reproductive capacity of the planarians starts to be damaged from the 17th to 25th days and to disappear completely from the 26th to 37th days during starvation. The expression patterns of the four genes exhibit the obvious dynamic variations during their desexualization, which indicates that these genes might be involved in gonad development.Sociedade Brasileira de Zoologia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100335Zoologia (Curitiba) v.35 2018reponame:Zoologia (Curitiba. Online)instname:Sociedade Brasileira de Zoologiainstacron:SBZ10.3897/zoologia.35.e21933info:eu-repo/semantics/openAccessDong,ZimeiShi,ChangyingChu,GengboDong,YanpingChen,GuangwenLiu,Dezengeng2018-09-05T00:00:00Zoai:scielo:S1984-46702018000100335Revistahttp://www.scielo.br/zoolONGhttps://old.scielo.br/oai/scielo-oai.phpsbz@sbzoologia.org.br1984-46891984-4670opendoar:2018-09-05T00:00Zoologia (Curitiba. Online) - Sociedade Brasileira de Zoologiafalse |
dc.title.none.fl_str_mv |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
title |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
spellingShingle |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) Dong,Zimei Planarian food deprivation germ cells reproductive system |
title_short |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
title_full |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
title_fullStr |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
title_full_unstemmed |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
title_sort |
Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) |
author |
Dong,Zimei |
author_facet |
Dong,Zimei Shi,Changying Chu,Gengbo Dong,Yanping Chen,Guangwen Liu,Dezeng |
author_role |
author |
author2 |
Shi,Changying Chu,Gengbo Dong,Yanping Chen,Guangwen Liu,Dezeng |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dong,Zimei Shi,Changying Chu,Gengbo Dong,Yanping Chen,Guangwen Liu,Dezeng |
dc.subject.por.fl_str_mv |
Planarian food deprivation germ cells reproductive system |
topic |
Planarian food deprivation germ cells reproductive system |
description |
ABSTRACT Planarians, the representatives of an ancient bilaterian group with complex reproductive system and high regenerative capabilities, are model system suitable for studying the basic molecular requirements for the development of the reproductive system. To further explore the morphological changes of the gonads during desexualization and the molecular events of the genes controlling the reproductive system development in planarians, we have investigated the histological changes of ovary and testis by paraffin section and the expression patterns of reproductive-related genes by the quantitative real-time PCR in Dugesia japonica Ichikawa & Kawakatsu, 1964, upon starvation. The four genes, Djprps, DjvlgA, DjvlgB and Djnos, have been selected. The research results show that the degradation of ovary changes from outside layer to inside, and the testis changes are opposite; the reproductive capacity of the planarians starts to be damaged from the 17th to 25th days and to disappear completely from the 26th to 37th days during starvation. The expression patterns of the four genes exhibit the obvious dynamic variations during their desexualization, which indicates that these genes might be involved in gonad development. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100335 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100335 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3897/zoologia.35.e21933 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
dc.source.none.fl_str_mv |
Zoologia (Curitiba) v.35 2018 reponame:Zoologia (Curitiba. Online) instname:Sociedade Brasileira de Zoologia instacron:SBZ |
instname_str |
Sociedade Brasileira de Zoologia |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Zoologia (Curitiba. Online) |
collection |
Zoologia (Curitiba. Online) |
repository.name.fl_str_mv |
Zoologia (Curitiba. Online) - Sociedade Brasileira de Zoologia |
repository.mail.fl_str_mv |
sbz@sbzoologia.org.br |
_version_ |
1750318092549881856 |