Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/11824 |
Resumo: | The autonomic nervous system (ANS) regulate and coordinate processes and function of organs to maintain homeostasis of the cardiovascular system in response of changes in the internal environment. Frequently, the understanding of the response patterns, interaction reflexes and other factors regulated by ANS, required in vivo studies. The use of anesthetics during the course of experimental protocols and data collection has been a way of solving the problems related of such studies. However, anesthetics depress the nervous system and compromise the conclusions from investigations that rely on functional ANS. Thus, in this work, we sought to validate a decerebrated preparation with individuals of the species Crotalus durissus. Decerebration consists in the ablation of superior brain areas in order to maintain preparations that are incapable of superior processing, perception and sensations of any type, including stress or pain. In these preparations, areas of the brainstem and part of the hypothalamus are maintained. Thus, the cardiovascular homeostasis is maintained. In the present study, we performed series of physiological tests for validated the presence of autonomic responses in the model. In addition, we performed a baroreflex study to understand how effective is the model in representing the intact animal. Decerebrated animals were able to keep hemodynamic variables constant during the tail lift protocol. In the head lift maneuver, the systemic mean arterial pressure (MAP sys) increased to ensure adequate blood supply to the head. In addition, during surface heat application, the heart rate (fH) increased, a cardiovascular response typical of Squamata group members during heating. Pharmacological stimulation of O₂ chemoreceptors induced elevations in fH and changes in net shunt. These tests attest that diverse populations of baroreceptors, O₂ chemoreceptors and temperature sensors have functional afferences generating autonomic reflexes even after the decerebration procedure. This indicates that the ANS of the decerebrated animals remains functional and capable of performing hemodynamic compensations to various perturbations. The study of the baroreflex through the pharmacological method indicated that the decerebrated animals maintain the gain and baroreflex limits similar to intact animals. We conclude that the decerebration doesn’t eliminate the reflexes generated by the centers of cardiovascular integration in the central nervous system (CNS) of rattlesnakes, keeping it functional like intact animals. In addition, isoflurane anesthesia suppresses the baroreflex of the decerebrated animals, modify the responses to autonomic function protocols, and causes a decrease of the mean arterial pressure. Thus, the use of anesthesia in the course of experimental protocols involving autonomic reflexes is not indicated. |
id |
SCAR_1a385e82b41c82cb05c4c132a3c62bac |
---|---|
oai_identifier_str |
oai:repositorio.ufscar.br:ufscar/11824 |
network_acronym_str |
SCAR |
network_name_str |
Repositório Institucional da UFSCAR |
repository_id_str |
4322 |
spelling |
Rocha, Gabriella CamposLeite, Cléo Alcantara Costahttp://lattes.cnpq.br/2031764784233552http://lattes.cnpq.br/1305264011164154dccdbfab-80c1-4d30-b216-e8d5b8fae0f32019-09-12T17:39:49Z2019-09-12T17:39:49Z2019-05-24ROCHA, Gabriella Campos. Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus). 2019. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11824.https://repositorio.ufscar.br/handle/ufscar/11824The autonomic nervous system (ANS) regulate and coordinate processes and function of organs to maintain homeostasis of the cardiovascular system in response of changes in the internal environment. Frequently, the understanding of the response patterns, interaction reflexes and other factors regulated by ANS, required in vivo studies. The use of anesthetics during the course of experimental protocols and data collection has been a way of solving the problems related of such studies. However, anesthetics depress the nervous system and compromise the conclusions from investigations that rely on functional ANS. Thus, in this work, we sought to validate a decerebrated preparation with individuals of the species Crotalus durissus. Decerebration consists in the ablation of superior brain areas in order to maintain preparations that are incapable of superior processing, perception and sensations of any type, including stress or pain. In these preparations, areas of the brainstem and part of the hypothalamus are maintained. Thus, the cardiovascular homeostasis is maintained. In the present study, we performed series of physiological tests for validated the presence of autonomic responses in the model. In addition, we performed a baroreflex study to understand how effective is the model in representing the intact animal. Decerebrated animals were able to keep hemodynamic variables constant during the tail lift protocol. In the head lift maneuver, the systemic mean arterial pressure (MAP sys) increased to ensure adequate blood supply to the head. In addition, during surface heat application, the heart rate (fH) increased, a cardiovascular response typical of Squamata group members during heating. Pharmacological stimulation of O₂ chemoreceptors induced elevations in fH and changes in net shunt. These tests attest that diverse populations of baroreceptors, O₂ chemoreceptors and temperature sensors have functional afferences generating autonomic reflexes even after the decerebration procedure. This indicates that the ANS of the decerebrated animals remains functional and capable of performing hemodynamic compensations to various perturbations. The study of the baroreflex through the pharmacological method indicated that the decerebrated animals maintain the gain and baroreflex limits similar to intact animals. We conclude that the decerebration doesn’t eliminate the reflexes generated by the centers of cardiovascular integration in the central nervous system (CNS) of rattlesnakes, keeping it functional like intact animals. In addition, isoflurane anesthesia suppresses the baroreflex of the decerebrated animals, modify the responses to autonomic function protocols, and causes a decrease of the mean arterial pressure. Thus, the use of anesthesia in the course of experimental protocols involving autonomic reflexes is not indicated.A coordenação de processos e funcionamento de órgãos para manutenção da homeostase do sistema cardiovascular diante de alterações do meio interno é regulada em parte pelo sistema nervoso autônomo (SNA). Muitas vezes a compreensão dos mecanismos regulados pelo SNA, padrões de resposta, interação entre reflexos, dentre diversos outros fatores, requer estudos in vivo. O uso de anestésicos durante o curso dos protocolos experimentais e coleta de dados tem sido uma maneira de resolver os problemas éticos relacionados ao grau de instrumentação necessário para tais estudos. Os anestésicos, no entanto, deprimem o sistema nervoso e comprometem a conclusão de investigações que dependem do SNA funcional. Dessa maneira, nesse trabalho, buscamos validar uma preparação descerebrada com indivíduos da espécie Crotalus durissus. A descerebração consiste na inviabilização de áreas encefálicas superiores a fim de manter animais incapazes de processamento superior de informações e inviabilizando percepção e sensações de quaisquer tipos, incluindo estresse ou dor. Nestes animais, áreas do tronco encefálico e parte do hipotálamo, que participam da homeostase cardiovascular, são mantidas. No presente estudo, realizamos séries de testes fisiológicos para validação da presença de respostas autonômicas no modelo descerebrado. Além disso, realizamos o estudo do barorreflexo para compreensão de quão efetivo é o modelo em representar o animal intacto. Os animais descerebrados foram capazes de manter as variáveis hemodinâmicas constantes durante o protocolo de levantamento da cauda. Na manobra de levantamento da cabeça a pressão arterial média sistêmica (PAM sis) se elevou para garantir o suprimento adequado de sangue na cabeça. Além disso, durante aplicação de calor superficial, a frequência cardíaca (fH) se elevou, uma resposta cardiovascular típica de membros do grupo Squamata durante o aquecimento. A estimulação farmacológica de quimiorreceptores de O₂ através de injeções de cianeto de sódio produziu elevações na (fH) e alterações do net shunt. Estes testes atestam que diversas populações de barorreceptores, quimiorreceptores de O₂ e sensores de temperatura superficial possuem aferências funcionais gerando reflexos autonômicos esperados mesmo após o procedimento de descerebração. Isso indica que o SNA dos animais descerebrados se mantém funcional e capaz de executar compensações hemodinâmicas à diversas perturbações. O estudo do barorreflexo foi realizado em animais descerebrados e intactos. Através do método farmacológico, observamos que os animais descerebrados mantêm o ganho e os limites do barorreflexo similar à animais intactos. Com isso concluímos que a descerebração não elimina os reflexos gerados pelos centros de integração cardiovascular no sistema nervoso central (SNC) das cascavéis, mantendo-o com funcionamento equivalente ao animal intacto, mesmo sem as interações superiores. Além disso, a anestesia com isoflurano suprime as respostas barorreflexas dos animais descerebrados e altera as respostas aos protocolos de função autonômica, além de causar diminuição da Pa média dos animais. Assim, o uso da anestesia no curso de protocolos experimentais envolvendo reflexos autonômicos não é indicada.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CAPES: 1692101FAPESP: 2018/05035-0porUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarAquecimentoBarorreflexoCascavelDescerebraçãoDesvios intracardíacosHipóxiaMétodo da sequênciaBaroreflexDecerebrationRattlesnakeHeatingHypoxiaNet shuntSequence methodCIENCIAS BIOLOGICAS::FISIOLOGIAAvaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus)Evaluation of analysis methods for assessment of the baroreflex sensitivity and one study model with the South American rattlesnake (Crotalus durissus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis24 meses após a data da defesad8186c0c-8757-48e5-ada9-61d1171ae48finfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdfDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdfDissertação de mestrado Gabriella Campos Rochaapplication/pdf2395151https://repositorio.ufscar.br/bitstream/ufscar/11824/2/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf3addd4e504b81b715e6fb03d9899beffMD52VERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdfVERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdfVersão parcial da dissertação de mestrado Gabriella Campos Rochaapplication/pdf685583https://repositorio.ufscar.br/bitstream/ufscar/11824/3/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf971ef5fe38bbb241264735057ba1d089MD53Carta comprovante repositorio Gabriella Campos Rocha.pdfCarta comprovante repositorio Gabriella Campos Rocha.pdfCarta comprovanteapplication/pdf610010https://repositorio.ufscar.br/bitstream/ufscar/11824/4/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf3148937b2f864cea9c795bf51bc8768bMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/11824/5/license.txtae0398b6f8b235e40ad82cba6c50031dMD55TEXTDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdf.txtDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdf.txtExtracted texttext/plain208685https://repositorio.ufscar.br/bitstream/ufscar/11824/6/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf.txt85d2b39386849f2334dcd3b6a197bb29MD56VERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdf.txtVERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdf.txtExtracted texttext/plain103239https://repositorio.ufscar.br/bitstream/ufscar/11824/8/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf.txtd7cad054135c88b30a08efec58493f52MD58Carta comprovante repositorio Gabriella Campos Rocha.pdf.txtCarta comprovante repositorio Gabriella Campos Rocha.pdf.txtExtracted texttext/plain1https://repositorio.ufscar.br/bitstream/ufscar/11824/10/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf.txt68b329da9893e34099c7d8ad5cb9c940MD510THUMBNAILDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdf.jpgDISSERTAÇÃO DE MESTRADO GABRIELLA C ROCHA.pdf.jpgIM Thumbnailimage/jpeg5061https://repositorio.ufscar.br/bitstream/ufscar/11824/7/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf.jpg9038a3db804481e45285d179c4baffd4MD57VERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdf.jpgVERSÃO PARCIAL DISSERTAÇÃO DE MESTRADO GABRIELLA CAMPOS ROCHA.pdf.jpgIM Thumbnailimage/jpeg5061https://repositorio.ufscar.br/bitstream/ufscar/11824/9/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf.jpg9038a3db804481e45285d179c4baffd4MD59Carta comprovante repositorio Gabriella Campos Rocha.pdf.jpgCarta comprovante repositorio Gabriella Campos Rocha.pdf.jpgIM Thumbnailimage/jpeg13192https://repositorio.ufscar.br/bitstream/ufscar/11824/11/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf.jpgedd54755e8c413de14fb06981549c5e5MD511ufscar/118242023-09-18 18:31:44.529oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:44Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
dc.title.alternative.eng.fl_str_mv |
Evaluation of analysis methods for assessment of the baroreflex sensitivity and one study model with the South American rattlesnake (Crotalus durissus) |
title |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
spellingShingle |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) Rocha, Gabriella Campos Aquecimento Barorreflexo Cascavel Descerebração Desvios intracardíacos Hipóxia Método da sequência Baroreflex Decerebration Rattlesnake Heating Hypoxia Net shunt Sequence method CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
title_full |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
title_fullStr |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
title_full_unstemmed |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
title_sort |
Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus) |
author |
Rocha, Gabriella Campos |
author_facet |
Rocha, Gabriella Campos |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/1305264011164154 |
dc.contributor.author.fl_str_mv |
Rocha, Gabriella Campos |
dc.contributor.advisor1.fl_str_mv |
Leite, Cléo Alcantara Costa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2031764784233552 |
dc.contributor.authorID.fl_str_mv |
dccdbfab-80c1-4d30-b216-e8d5b8fae0f3 |
contributor_str_mv |
Leite, Cléo Alcantara Costa |
dc.subject.por.fl_str_mv |
Aquecimento Barorreflexo Cascavel Descerebração Desvios intracardíacos Hipóxia Método da sequência |
topic |
Aquecimento Barorreflexo Cascavel Descerebração Desvios intracardíacos Hipóxia Método da sequência Baroreflex Decerebration Rattlesnake Heating Hypoxia Net shunt Sequence method CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Baroreflex Decerebration Rattlesnake Heating Hypoxia Net shunt Sequence method |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
The autonomic nervous system (ANS) regulate and coordinate processes and function of organs to maintain homeostasis of the cardiovascular system in response of changes in the internal environment. Frequently, the understanding of the response patterns, interaction reflexes and other factors regulated by ANS, required in vivo studies. The use of anesthetics during the course of experimental protocols and data collection has been a way of solving the problems related of such studies. However, anesthetics depress the nervous system and compromise the conclusions from investigations that rely on functional ANS. Thus, in this work, we sought to validate a decerebrated preparation with individuals of the species Crotalus durissus. Decerebration consists in the ablation of superior brain areas in order to maintain preparations that are incapable of superior processing, perception and sensations of any type, including stress or pain. In these preparations, areas of the brainstem and part of the hypothalamus are maintained. Thus, the cardiovascular homeostasis is maintained. In the present study, we performed series of physiological tests for validated the presence of autonomic responses in the model. In addition, we performed a baroreflex study to understand how effective is the model in representing the intact animal. Decerebrated animals were able to keep hemodynamic variables constant during the tail lift protocol. In the head lift maneuver, the systemic mean arterial pressure (MAP sys) increased to ensure adequate blood supply to the head. In addition, during surface heat application, the heart rate (fH) increased, a cardiovascular response typical of Squamata group members during heating. Pharmacological stimulation of O₂ chemoreceptors induced elevations in fH and changes in net shunt. These tests attest that diverse populations of baroreceptors, O₂ chemoreceptors and temperature sensors have functional afferences generating autonomic reflexes even after the decerebration procedure. This indicates that the ANS of the decerebrated animals remains functional and capable of performing hemodynamic compensations to various perturbations. The study of the baroreflex through the pharmacological method indicated that the decerebrated animals maintain the gain and baroreflex limits similar to intact animals. We conclude that the decerebration doesn’t eliminate the reflexes generated by the centers of cardiovascular integration in the central nervous system (CNS) of rattlesnakes, keeping it functional like intact animals. In addition, isoflurane anesthesia suppresses the baroreflex of the decerebrated animals, modify the responses to autonomic function protocols, and causes a decrease of the mean arterial pressure. Thus, the use of anesthesia in the course of experimental protocols involving autonomic reflexes is not indicated. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-09-12T17:39:49Z |
dc.date.available.fl_str_mv |
2019-09-12T17:39:49Z |
dc.date.issued.fl_str_mv |
2019-05-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROCHA, Gabriella Campos. Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus). 2019. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11824. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/11824 |
identifier_str_mv |
ROCHA, Gabriella Campos. Avaliação da viabilidade de métodos de análise da sensibilidade barorreflexa e de um modelo de estudo com a cascavel sul americana (Crotalus durissus). 2019. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11824. |
url |
https://repositorio.ufscar.br/handle/ufscar/11824 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.authority.fl_str_mv |
d8186c0c-8757-48e5-ada9-61d1171ae48f |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
dc.publisher.initials.fl_str_mv |
UFSCar |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFSCAR instname:Universidade Federal de São Carlos (UFSCAR) instacron:UFSCAR |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
UFSCAR |
institution |
UFSCAR |
reponame_str |
Repositório Institucional da UFSCAR |
collection |
Repositório Institucional da UFSCAR |
bitstream.url.fl_str_mv |
https://repositorio.ufscar.br/bitstream/ufscar/11824/2/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf https://repositorio.ufscar.br/bitstream/ufscar/11824/3/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf https://repositorio.ufscar.br/bitstream/ufscar/11824/4/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf https://repositorio.ufscar.br/bitstream/ufscar/11824/5/license.txt https://repositorio.ufscar.br/bitstream/ufscar/11824/6/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/11824/8/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/11824/10/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/11824/7/DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20C%20ROCHA.pdf.jpg https://repositorio.ufscar.br/bitstream/ufscar/11824/9/VERS%c3%83O%20PARCIAL%20DISSERTA%c3%87%c3%83O%20DE%20MESTRADO%20GABRIELLA%20CAMPOS%20ROCHA.pdf.jpg https://repositorio.ufscar.br/bitstream/ufscar/11824/11/Carta%20comprovante%20repositorio%20Gabriella%20Campos%20Rocha.pdf.jpg |
bitstream.checksum.fl_str_mv |
3addd4e504b81b715e6fb03d9899beff 971ef5fe38bbb241264735057ba1d089 3148937b2f864cea9c795bf51bc8768b ae0398b6f8b235e40ad82cba6c50031d 85d2b39386849f2334dcd3b6a197bb29 d7cad054135c88b30a08efec58493f52 68b329da9893e34099c7d8ad5cb9c940 9038a3db804481e45285d179c4baffd4 9038a3db804481e45285d179c4baffd4 edd54755e8c413de14fb06981549c5e5 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
|
_version_ |
1802136363811733504 |