Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa

Detalhes bibliográficos
Autor(a) principal: Cavicchioli, Rafael
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/15644
Resumo: Endophytes in general are among the most productive sources of secondary metabolites, presenting a series of biological activities and applications. It is estimated that approximately half of the antibiotics produced industrially, in addition to several other compounds, are produced by these organisms. According to the World Health Organization (WHO) 90% of bacterial strains are resistant to first-choice drugs. Therefore, the search for new molecules with antimicrobial activity is urgent. In view of this, the present study sought to optimize cultivation conditions for the production of fermented broth in order to achieve the greatest amount and diversity of secondary metabolites with biological action. Different types of cultures (axenic and mixed) were tested in order to evaluate their effectiveness in relation to: antimicrobial activity, leishmanicidal activity, antitumor activity and cytotoxicity. Based on the data obtained, a time of 168 hours was established for the production of broths. The mixed culture showed greater activity than the axenic culture in relation to antimicrobial activity, establishing 95.86% of inhibition at its lowest concentration tested for P. aeruginosa, 92.86% of inhibition against E.coli strains, 91.13% against Salmonella setubal. Using a concentration of 2500 µg, an inhibition of 82.5% was obtained in S. aureus and 86% in S. epidermidis. Regarding the leishmanicidal activity, the mixed culture broth showed a greater action than the simple culture when analyzed at lower concentrations. Cytotoxicity tests demonstrate that they are toxic only from a concentration of 10000 µg. From these results we can conclude that mixed cultures have great biological potential, in non-toxic concentrations. Multidrug resistance occurs in both Gram-positive and Gram-negative bacteria, which cause infections that cannot be treated with most conventional antibiotics. Due to these and other factors, microbial resistance is considered an alarming public health problem, showing an increasing number of infections caused by resistant bacteria, which cause infections in hospitals and communities. When some type of infection can no longer be treated by first-line antibiotics, usually more expensive drugs must be selected for treatment. The longer duration of the illness and treatment in question, often in the hospitals themselves, increases health care costs. A report citing the top threats to antibiotic resistance from the CDC in the United States (2019 Antibiotic Resistance Threats Report) includes the latest national estimates of deaths and infections that highlight the ongoing threat of antibiotic resistance. According to the report, more than 2.8 million antibiotic-resistant infections occur in the US each year, and more than 35,000 people die as a result. Based on these and other data, the second part of the study aims to find strains with antimicrobial activity from the plant species Coffea arabica and Xylopia aromatica and selects 6 strains that have great biotechnological potential. These strains were isolated in later studies and showed considerable antimicrobial activity.
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spelling Cavicchioli, RafaelSousa, Cristina Paiva dehttp://lattes.cnpq.br/9002619114161319Aníbal, Fernanda de Freitashttp://lattes.cnpq.br/4918261968772806http://lattes.cnpq.br/3348518666934464fd5ab1af-becc-402a-b6c1-227498e2e0d32022-02-23T19:36:01Z2022-02-23T19:36:01Z2021-08-20CAVICCHIOLI, Rafael. Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa. 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15644.https://repositorio.ufscar.br/handle/ufscar/15644Endophytes in general are among the most productive sources of secondary metabolites, presenting a series of biological activities and applications. It is estimated that approximately half of the antibiotics produced industrially, in addition to several other compounds, are produced by these organisms. According to the World Health Organization (WHO) 90% of bacterial strains are resistant to first-choice drugs. Therefore, the search for new molecules with antimicrobial activity is urgent. In view of this, the present study sought to optimize cultivation conditions for the production of fermented broth in order to achieve the greatest amount and diversity of secondary metabolites with biological action. Different types of cultures (axenic and mixed) were tested in order to evaluate their effectiveness in relation to: antimicrobial activity, leishmanicidal activity, antitumor activity and cytotoxicity. Based on the data obtained, a time of 168 hours was established for the production of broths. The mixed culture showed greater activity than the axenic culture in relation to antimicrobial activity, establishing 95.86% of inhibition at its lowest concentration tested for P. aeruginosa, 92.86% of inhibition against E.coli strains, 91.13% against Salmonella setubal. Using a concentration of 2500 µg, an inhibition of 82.5% was obtained in S. aureus and 86% in S. epidermidis. Regarding the leishmanicidal activity, the mixed culture broth showed a greater action than the simple culture when analyzed at lower concentrations. Cytotoxicity tests demonstrate that they are toxic only from a concentration of 10000 µg. From these results we can conclude that mixed cultures have great biological potential, in non-toxic concentrations. Multidrug resistance occurs in both Gram-positive and Gram-negative bacteria, which cause infections that cannot be treated with most conventional antibiotics. Due to these and other factors, microbial resistance is considered an alarming public health problem, showing an increasing number of infections caused by resistant bacteria, which cause infections in hospitals and communities. When some type of infection can no longer be treated by first-line antibiotics, usually more expensive drugs must be selected for treatment. The longer duration of the illness and treatment in question, often in the hospitals themselves, increases health care costs. A report citing the top threats to antibiotic resistance from the CDC in the United States (2019 Antibiotic Resistance Threats Report) includes the latest national estimates of deaths and infections that highlight the ongoing threat of antibiotic resistance. According to the report, more than 2.8 million antibiotic-resistant infections occur in the US each year, and more than 35,000 people die as a result. Based on these and other data, the second part of the study aims to find strains with antimicrobial activity from the plant species Coffea arabica and Xylopia aromatica and selects 6 strains that have great biotechnological potential. These strains were isolated in later studies and showed considerable antimicrobial activity.Endofíticos em geral encontram-se entre as fontes mais produtivas de metabólitos secundários, apresentando uma série de atividades biológicas e aplicações. Estima-se que aproximadamente metade dos antibióticos produzidos industrialmente, além de outros diversos compostos sejam produzidos por esses organismos. Segundo a Organização Mundial da Saúde (OMS) 90% das linhagens bacterianas são resistentes a drogas de primeira escolha. Portanto, a busca por novas moléculas com atividade antimicrobiana é urgente. Em vista disso, o presente estudo buscou otimizar condições de cultivo para a produção do caldo fermentado visando atingir a maior quantidade e diversidade de metabólitos secundários com ação biológica. Foram testados diferentes tipos de cultivos (axênicos e mistos), com a finalidade de avaliar a eficácia dos mesmos em relação à: atividade antimicrobiana, atividade leishmanicida, atividade antitumoral e citotoxicidade. Com base nos dados obtidos foi estabelecido um tempo de 168 horas para a produção dos caldos. O cultivo misto demonstrou maior atividade que o cultivo axênico em relação a atividade antimicrobiana, estabelecendo 95,86% de inibição em sua menor concentração testada para P. aeruginosa, 92,86% de inibição contra cepas de E.coli, 91,13% contra cepas de Salmonella. Utilizando a concentração de 2500 µg, obteve-se uma inibição de 82,5% em S. aureus e de 86% em S. epidermidis. Se tratando da atividade leishmanicida o caldo de cultivo misto demonstrou uma maior ação do que o de cultivo simples quando analisado em menores concentrações. Os testes de citotoxicidade demonstram uma serem tóxicos apenas a partir da concentração de 10000 µg. A partir desses resultados podemos concluir que os cultivos mistos apresentam grande potencial biológico, em concentrações não tóxicas. A resistência a múltiplas drogas ocorre em bactérias GRAM-positivas e Gram-negativas, que causam infecções das quais não podem ser tratadas com a maioria dos antibióticos convencionais. Devido a esses e outros fatores a resistência microbiana é considerado um problema de saúde pública alarmante mostrando um número crescente de infecções causadas por bactérias resistentes, que causam infecções em hospitais e comunidades. Quando algum tipo de infecção já não pode ser tratado por antibióticos de primeira linha, geralmente medicamentos mais caros devem ser selecionados para o tratamento. A duração maior da doença e do tratamento em questão, muitas vezes nos próprios hospitais, aumenta os custos com cuidados de saúde. Um relatório citando as principais ameaças à resistência a antibióticos, do CDC nos Estados Unidos (Relatório de ameaças à Resistencia a Antibióticos de 2019), incluem as mais recentes estimativas nacionais de mortes e infecções que destacam a ameaça contínua de resistência a antibióticos. De acordo com o relatório, mais de 2,8 milhões de infecções resistentes a antibióticos ocorrem nos EUA a cada ano, e mais de 35.000 pessoas morrem como resultado. Com base nesses e em outros dados, a segunda parte do estudo tem como objetivo encontrar cepas com atividade antimicrobiana oriundas das espécies vegetais Coffea arabica e Xylopia aromatica e seleciona 6 cepas que apresentam grande potencial biotecnológico. Estas cepas foram isoladas em estudos posteriores e apresentaram atividade antimicrobiana considerável.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)88882.426500/2019-01porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessEndofíticosAtividade biológicaCitotoxicidadeEndophytesBiological activityCytotoxicityCIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA::MICROBIOLOGIA INDUSTRIAL E DE FERMENTACAOBioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxaBioprospection of new isolates and evaluation of the antimicrobial, leishmanicidal, antitumor potential and cytotoxic profile of pure and mixed cultures of Paenibacillus polymyxainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis60060035b91dbf-6130-418b-915b-3380dadd62dcreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15644/5/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD55ORIGINALTese Rafael+Cavicchioli.pdfTese Rafael+Cavicchioli.pdfTese de Doutoradoapplication/pdf3243116https://repositorio.ufscar.br/bitstream/ufscar/15644/3/Tese%20Rafael%2bCavicchioli.pdffc0982ca2ddf9e9fc241312e5559eca9MD53Carta Comprovante - Orientador.pdfCarta Comprovante - Orientador.pdfCarta comprovante orientadorapplication/pdf173179https://repositorio.ufscar.br/bitstream/ufscar/15644/4/Carta%20Comprovante%20-%20Orientador.pdf0ea3bef3c0a4c5ee5c20c09f0100ecd3MD54TEXTTese Rafael+Cavicchioli.pdf.txtTese Rafael+Cavicchioli.pdf.txtExtracted texttext/plain242279https://repositorio.ufscar.br/bitstream/ufscar/15644/6/Tese%20Rafael%2bCavicchioli.pdf.txt7e339ea886726e15bcb14cb4d4c9b29dMD56Carta Comprovante - Orientador.pdf.txtCarta Comprovante - Orientador.pdf.txtExtracted texttext/plain1651https://repositorio.ufscar.br/bitstream/ufscar/15644/8/Carta%20Comprovante%20-%20Orientador.pdf.txtdc2e16bb6b8e7fb251edf7501cc712d6MD58THUMBNAILTese Rafael+Cavicchioli.pdf.jpgTese Rafael+Cavicchioli.pdf.jpgIM Thumbnailimage/jpeg5630https://repositorio.ufscar.br/bitstream/ufscar/15644/7/Tese%20Rafael%2bCavicchioli.pdf.jpg1b2f655f8cc8ce4f32cc13a7151c642dMD57Carta Comprovante - Orientador.pdf.jpgCarta Comprovante - Orientador.pdf.jpgIM Thumbnailimage/jpeg6074https://repositorio.ufscar.br/bitstream/ufscar/15644/9/Carta%20Comprovante%20-%20Orientador.pdf.jpg439d6e43c896aed76034eaa0b95fe8d9MD59ufscar/156442023-09-18 18:32:25.995oai:repositorio.ufscar.br:ufscar/15644Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:25Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
dc.title.alternative.eng.fl_str_mv Bioprospection of new isolates and evaluation of the antimicrobial, leishmanicidal, antitumor potential and cytotoxic profile of pure and mixed cultures of Paenibacillus polymyxa
title Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
spellingShingle Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
Cavicchioli, Rafael
Endofíticos
Atividade biológica
Citotoxicidade
Endophytes
Biological activity
Cytotoxicity
CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA::MICROBIOLOGIA INDUSTRIAL E DE FERMENTACAO
title_short Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
title_full Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
title_fullStr Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
title_full_unstemmed Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
title_sort Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa
author Cavicchioli, Rafael
author_facet Cavicchioli, Rafael
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/3348518666934464
dc.contributor.author.fl_str_mv Cavicchioli, Rafael
dc.contributor.advisor1.fl_str_mv Sousa, Cristina Paiva de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9002619114161319
dc.contributor.advisor-co1.fl_str_mv Aníbal, Fernanda de Freitas
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4918261968772806
dc.contributor.authorID.fl_str_mv fd5ab1af-becc-402a-b6c1-227498e2e0d3
contributor_str_mv Sousa, Cristina Paiva de
Aníbal, Fernanda de Freitas
dc.subject.por.fl_str_mv Endofíticos
Atividade biológica
Citotoxicidade
topic Endofíticos
Atividade biológica
Citotoxicidade
Endophytes
Biological activity
Cytotoxicity
CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA::MICROBIOLOGIA INDUSTRIAL E DE FERMENTACAO
dc.subject.eng.fl_str_mv Endophytes
Biological activity
Cytotoxicity
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA::MICROBIOLOGIA INDUSTRIAL E DE FERMENTACAO
description Endophytes in general are among the most productive sources of secondary metabolites, presenting a series of biological activities and applications. It is estimated that approximately half of the antibiotics produced industrially, in addition to several other compounds, are produced by these organisms. According to the World Health Organization (WHO) 90% of bacterial strains are resistant to first-choice drugs. Therefore, the search for new molecules with antimicrobial activity is urgent. In view of this, the present study sought to optimize cultivation conditions for the production of fermented broth in order to achieve the greatest amount and diversity of secondary metabolites with biological action. Different types of cultures (axenic and mixed) were tested in order to evaluate their effectiveness in relation to: antimicrobial activity, leishmanicidal activity, antitumor activity and cytotoxicity. Based on the data obtained, a time of 168 hours was established for the production of broths. The mixed culture showed greater activity than the axenic culture in relation to antimicrobial activity, establishing 95.86% of inhibition at its lowest concentration tested for P. aeruginosa, 92.86% of inhibition against E.coli strains, 91.13% against Salmonella setubal. Using a concentration of 2500 µg, an inhibition of 82.5% was obtained in S. aureus and 86% in S. epidermidis. Regarding the leishmanicidal activity, the mixed culture broth showed a greater action than the simple culture when analyzed at lower concentrations. Cytotoxicity tests demonstrate that they are toxic only from a concentration of 10000 µg. From these results we can conclude that mixed cultures have great biological potential, in non-toxic concentrations. Multidrug resistance occurs in both Gram-positive and Gram-negative bacteria, which cause infections that cannot be treated with most conventional antibiotics. Due to these and other factors, microbial resistance is considered an alarming public health problem, showing an increasing number of infections caused by resistant bacteria, which cause infections in hospitals and communities. When some type of infection can no longer be treated by first-line antibiotics, usually more expensive drugs must be selected for treatment. The longer duration of the illness and treatment in question, often in the hospitals themselves, increases health care costs. A report citing the top threats to antibiotic resistance from the CDC in the United States (2019 Antibiotic Resistance Threats Report) includes the latest national estimates of deaths and infections that highlight the ongoing threat of antibiotic resistance. According to the report, more than 2.8 million antibiotic-resistant infections occur in the US each year, and more than 35,000 people die as a result. Based on these and other data, the second part of the study aims to find strains with antimicrobial activity from the plant species Coffea arabica and Xylopia aromatica and selects 6 strains that have great biotechnological potential. These strains were isolated in later studies and showed considerable antimicrobial activity.
publishDate 2021
dc.date.issued.fl_str_mv 2021-08-20
dc.date.accessioned.fl_str_mv 2022-02-23T19:36:01Z
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dc.identifier.citation.fl_str_mv CAVICCHIOLI, Rafael. Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa. 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15644.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/15644
identifier_str_mv CAVICCHIOLI, Rafael. Bioprospecção de novos isolados e avaliação do potencial antimicrobiano, leishmanicida, antitumoral e perfil citotóxico dos cultivos puros e mistos de Paenibacillus polymyxa. 2021. Tese (Doutorado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15644.
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